{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:42:06Z","timestamp":1787013726014,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Oxidative stress is a crucial pathological basis for diverse intestinal diseases, such as inflammatory bowel disease (IBD). Previous studies show that\n                      <jats:italic>Lactobacillus johnsonii<\/jats:italic>\n                      RS\u20107 alleviates colitis in mice, but its effect on intestinal oxidative stress remains unclear. Considering this, the research aimed to explore the role and mechanism of this strain in alleviating oxidative damage in a colitis model. Colitis was induced in the mice using dextran sulfate sodium (DSS), and mice were subsequently randomly assigned to four groups.\n                      <jats:italic>L. johnsonii<\/jats:italic>\n                      RS\u20107 was evaluated for its effects on colon damage, oxidative stress, the Nrf2 pathway, and intestinal metabolites using histopathology, biochemical assays, qPCR, Western blotting, and non\u2010targeted metabolomics. The results showed that\n                      <jats:italic>L. johnsonii<\/jats:italic>\n                      RS\u20107 significantly attenuated DSS\u2010induced colonic tissue injury and reversed the DSS\u2010associated decreases in serum CAT, T\u2010SOD, GSH, and T\u2010AOC levels (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05), along with an increase in malondialdehyde (MDA) (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Moreover, the strain significantly mitigated the down\u2010regulation of antioxidant enzyme mRNA expression in colonic tissues (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Mechanistically,\n                      <jats:italic>L. johnsonii<\/jats:italic>\n                      RS\u20107 restored expression of Nrf2, NQO1, and HO\u20101 proteins in the colon and suppressed the up\u2010regulation of Keap1. Non\u2010targeted metabolomic analysis of intestinal contents further demonstrated that the strain significantly reduced levels of LPS 18:0 and triglycerides while concurrently increasing concentrations of DL\u2010lysine and 5\u2010hydroxylysine (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Correlation analysis indicated that LPS 18:0 and DL\u2010lysine might be key metabolites regulating oxidative stress. The above results suggest that\n                      <jats:italic>L. johnsonii<\/jats:italic>\n                      RS\u20107 can alleviate colonic oxidative damage by regulating metabolites to activate the Nrf2 pathway, providing theoretical support for applying this strain to prevent intestinal diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70701","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T10:56:21Z","timestamp":1763376981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Lactobacillus Johnsonii<\/i>\n                    RS\u20107 Prevents Nrf2\u2010Mediated Intestinal Oxidative Damage by Modulating Microbial Metabolites"],"prefix":"10.1111","volume":"90","author":[{"given":"Kai","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Life Sciences and Agri\u2010forestry Southwest University of Science and Technology  Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengfeng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Agri\u2010forestry Southwest University of Science and Technology  Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfei","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Agri\u2010forestry Southwest University of Science and Technology  Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanchuan","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Agri\u2010forestry Southwest University of Science and Technology  Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengxiao","family":"Zhai","sequence":"additional","affiliation":[{"name":"Tianfu Institute of Research and Innovation Southwest University of Science and Technology  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7844-4918","authenticated-orcid":false,"given":"Jingbo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Agri\u2010forestry Southwest University of Science and Technology  Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12040901"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2024.116197"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/app11104333"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15051127"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1756-4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i16.2841"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.022"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10121930"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13040573"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-017-0437-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9070610"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-020-05611-3"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu17010117"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/applmicrobiol3040081"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107696"},{"key":"e_1_2_10_17_1","first-page":"149","article-title":"Probiotics in Livestock and Poultry Nutrition and Health","author":"Mahesh M. 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