{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:24:15Z","timestamp":1773930255678,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T00:00:00Z","timestamp":1763856000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The excessive use of antibiotics in animal production has contributed to the global spread of antimicrobial resistance (AMR), posing a significant public health threat. To evaluate the antimicrobial susceptibility profiles and detect resistance genes in bacterial isolates from chicken, pork, and fish meat samples collected in the State of Paran\u00e1, Brazil, between 2017 and 2023. Meat samples (n = 418) were tested for\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp., while\n                      <jats:italic>Klebsiella<\/jats:italic>\n                      spp. (n = 265) and\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      spp. (n = 262) were included from 2019 onward. Bacterial isolation followed ISO 17604:2015 standards with MALDI\u2010TOF identification. Susceptibility testing was performed using the Kirby\u2010Bauer disk diffusion method. Detection of resistance genes (bla_TEM, bla_SHV, bla_CTX\u2010M, bla_KPC, bla_NDM, bla_OXA\u201048, bla_VIM, bla_SPM, and mcr\u20101) was conducted by real\u2010time PCR. Multidrug resistance (MDR) was widespread, particularly in\n                      <jats:italic>Salmonella spp<\/jats:italic>\n                      . and\n                      <jats:italic>Pseudomonas spp<\/jats:italic>\n                      ., followed by\n                      <jats:italic>Klebsiella spp<\/jats:italic>\n                      . and\n                      <jats:italic>E. coli<\/jats:italic>\n                      . Extended\u2010spectrum \u03b2\u2010lactamase (ESBL) genes were frequent in\n                      <jats:italic>E. coli<\/jats:italic>\n                      and\n                      <jats:italic>Klebsiella spp<\/jats:italic>\n                      ., while plasmid\u2010mediated colistin resistance (\n                      <jats:italic>mcr\u20101<\/jats:italic>\n                      ) and carbapenemase (\n                      <jats:italic>bla<\/jats:italic>\n                      KPC) were also identified. Resistance to third\u2010generation cephalosporins and ampicillin was especially high. Sampling across years and animal species may not fully reflect farm\u2010level resistance. The absence of molecular typing limited the ability to track clonal spread of resistant strains. Widespread AMR in foodborne bacteria was observed, with resistance levels often exceeding those reported in European monitoring. These findings underscore the need for strengthened surveillance and coordinated actions across animal and human health sectors.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70719","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T04:49:42Z","timestamp":1763959782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Monitoring of Antimicrobial Resistance Genes and Susceptibility Profiles in Bacterial Isolates From Animal\u2010Origin Meat"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6653-9952","authenticated-orcid":false,"given":"Andr\u00e9 Schenkel","family":"Dedecek","sequence":"first","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, School of Medicine Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"},{"name":"Laborat\u00f3rio Central do Estado do Paran\u00e1  S\u00e3o Jose dos Pinhais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4555-4632","authenticated-orcid":false,"given":"Lavinia","family":"Arend","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central do Estado do Paran\u00e1  S\u00e3o Jose dos Pinhais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margareth Leonor","family":"Penkal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central do Estado do Paran\u00e1  S\u00e3o Jose dos Pinhais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salesia Maria Prodocimo","family":"Moscardi","sequence":"additional","affiliation":[{"name":"Vigil\u00e2ncia Sanit\u00e1ria de Alimentos da Secretaria de Sa\u00fade do Estado do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3471-1786","authenticated-orcid":false,"given":"Felipe Francisco","family":"Tuon","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Infectious Diseases, School of Medicine Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115930"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2014.11.003"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04146\u201014"},{"key":"e_1_2_11_5_1","unstructured":"BRASIL ANVISA.2021.Projeto de Programa Nacional de Monitoramento de Microrganismos Resistentes e Res\u00edduos de Antimicrobianos em Alimentos\u2014Monitora Alimentos AMR. ANVISA.https:\/\/www.gov.br\/anvisa\/pt\u2010br\/assuntos\/fiscalizacao\u2010e\u2010monitoramento\/programas\u2010nacionais\u2010de\u2010monitoramento\u2010de\u2010alimentos\/monitoramento\u2010de\u2010microorganismos\u2010resistentes\u2010e\u2010residuos\u2010de\u2010antimicrobianos\u2010em\u2010alimentos."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2023.100580"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.101832"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(16)00056\u20106"},{"key":"e_1_2_11_9_1","unstructured":"EUCAST.2021.Clinical Breakpoint. European Committee on Antimicrobial Susceptibility Testing.https:\/\/www.eucast.org\/."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2024.8583"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4081\/ijfs.2022.9980"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12895"},{"key":"e_1_2_11_13_1","first-page":"13","article-title":"Investigation of Resistance of Salmonella spp. Isolated From Products and Raw Material of Animal Origin (Swine and Poultry) to Antibiotics and Disinfectants","volume":"20","author":"Maciel M. 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