{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:21:01Z","timestamp":1773930061138,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:sec>\n                    <jats:title>ABSTRACT<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Opuntia<\/jats:italic>\n                      plants are well\u2010known for their richness in numerous compounds with potential biological properties against human diseases, including ulcers, asthma, and hemorrhoids. This study aimed to evaluate the anticancer activity of different parts (seeds, pulp, and fruit) and extraction solvents of both cultivated and wild\n                      <jats:italic>Opuntia<\/jats:italic>\n                      fruits against various cell lines, as well as to identify the potential components responsible for this activity using a bioactivity\u2010based molecular networking approach with LC\u2010MS\/MS data. Among the different\n                      <jats:italic>Opuntia<\/jats:italic>\n                      extracts, the aqueous wild seeds (WS) exhibited a potent cytotoxic effect (&gt;35% of cytotoxicity) against the carcinogenic HCT\u2010116, MG\u201063, and T\u201047D cells with minor toxicity (&lt;15%) to immortalized non\u2010cancerogenic normal cells (hCMEC\/D3, HaCaT, and 3T3\u2010L1). A notable sensibility of the colon carcinoma cancer line (HCT\u2010116) was observed toward the aqueous WS, reducing their viability by 65.5% at the concentration of 1000\u00a0\u00b5g\/mL, with an IC\n                      <jats:sub>50<\/jats:sub>\n                      of 685.4 \u00b1 24.3\u00a0\u00b5g\/mL. This extract also displayed cytotoxic effects on HCT\u2010116 multicellular spheroids, impacting only the outer layer of the spheroids containing the active proliferating cells. The metabolite profiling by LC\u2010MS\/MS on the GNPS platform identified 79 mass peaks; among them, 18 were specific to the aqueous WS, which could be related to the observed cytotoxic activity. Among them, some compounds were already reported to have anticancer properties, namely, americanin, xanthoxylol, syringaldehyde, vanillin, and sinapaldehyde. To conclude,\n                      <jats:italic>Opuntia<\/jats:italic>\n                      seeds may constitute an attractive source of several promising anticancer agents, paving the way for potential pharmacological exploitation to treat colon carcinoma.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70771","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T09:43:36Z","timestamp":1765791816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolite Profiling of Bioactive Compounds in Algerian\n                    <i>Opuntia<\/i>\n                    Fruits With Emphasis on Their Anticancer Properties"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8146-1049","authenticated-orcid":false,"given":"Walid","family":"Zeghbib","sequence":"first","affiliation":[{"name":"Facult\u00e9 des Sciences de la Nature et de la Vie Laboratoire de Biochimie Appliqu\u00e9e Universit\u00e9 de Bejaia  Bejaia Algeria"},{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research Terminal de Cruzeiros do Porto de Leix\u00f5es  Matosinhos Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fares","family":"Boudjouan","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de Technologie Laboratoire de G\u00e9nie de l'Environnement Universit\u00e9 de Bejaia  Bejaia Algeria"},{"name":"Facult\u00e9 des Sciences de la Nature et de la Vie D\u00e9partement de Biotechnologie Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L\u00edgia","family":"Sousa","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research Terminal de Cruzeiros do Porto de Leix\u00f5es  Matosinhos Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma","family":"Ourabah","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences de la Nature et de la Vie Laboratoire de Biochimie Appliqu\u00e9e Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graciliana","family":"Lopes","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research Terminal de Cruzeiros do Porto de Leix\u00f5es  Matosinhos Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research Terminal de Cruzeiros do Porto de Leix\u00f5es  Matosinhos Portugal"},{"name":"FCUP\u2014Faculty of Sciences University of Porto  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph","family":"Urbatzka","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research Terminal de Cruzeiros do Porto de Leix\u00f5es  Matosinhos Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.15739\/IJAPR.20.001i"},{"issue":"7","key":"e_1_2_8_3_1","first-page":"1753","article-title":"Anti\u2010Cancer Activity of Opuntia Polyacantha Alkaloid Extract on Human Breast Cancer Cell Line","volume":"10","author":"Abdulazeem L.","year":"2018","journal-title":"Journal of Pharmaceutical Sciences and Research"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2020.21.10.3125"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130\u2010015\u20100505\u20106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010020\u20100317\u20105"},{"key":"e_1_2_8_7_1","first-page":"1","volume-title":"Genotoxicity\u2014A Predictable Risk to Our Actual World","author":"Aslant\u00fcrk \u00d6. 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