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This waste contains various bioactive molecules, such as collagen, which presents an increasing demand from the consumers. The narrownose smooth\u2010hound (\n                      <jats:italic>Mustelus schmitti<\/jats:italic>\n                      ) processing generates a large amount of residues. This study aimed to optimize the traditional collagen extraction process and evaluate alternative eco\u2010friendly collagen extraction techniques using the narrownose smooth\u2010hound skin as raw material. Results showed that the pre\u2010treatment optimal conditions were 2 h of deproteinization followed by 6 h of defatting. Acid extraction required 72 h to achieve maximum yield. Likewise, the maximum yield for the enzymatic extraction was obtained using 10% v\/v pepsin and 24 h of treatment. As an alternative technique, an enzymatic extraction was conducted using an endogenous enzyme obtained from the narrownose smooth\u2010hound stomach. This method acquired higher yields than the acid control and slightly lower yields than the commercial pepsin control. On the other hand, an acid extraction assisted with sonication was evaluated, with no significant differences observed. All extracts obtained were characterized by SDS\u2010PAGE, FTIR, UV\u2010spectra, and amino acid profile, confirming the presence of type I collagen. This study offers a sustainable solution to reduce fishery waste, generating two value\u2010added products, collagen and endogenous enzymes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>Optimization of collagen extraction from fisheries waste. Enzymatic extraction showed higher yields than the acidic one. Endogenous enzymes of fishery waste are a viable alternative for collagen extraction. The extracts obtained have characteristics that suggest type I collagen.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70828","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T16:48:30Z","timestamp":1767545310000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating Traditional and Alternative Eco\u2010Friendly Collagen Extraction Methods From Narrownose Smooth\u2010Hound Skin: A Sustainable Waste Reduction in the Fishing Industry"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2140-2488","authenticated-orcid":false,"given":"Victoria Florencia","family":"Alcolea Ersinger","sequence":"first","affiliation":[{"name":"National Institute of Fisheries Research and Development (INIDEP)  Mar del Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Lamas","sequence":"additional","affiliation":[{"name":"National Institute of Fisheries Research and Development (INIDEP)  Mar del Plata Argentina"},{"name":"Marine and Coastal Research Institute (IIM y C) National Research Council (CONICET)  Mar del Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Dell'Erba","sequence":"additional","affiliation":[{"name":"Chemistry and Biochemistry Department, Faculty of Exacts and Natural Sciences University of Mar del Plata  Mar del Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Cativa","sequence":"additional","affiliation":[{"name":"Institute of Materials Science and Technology (INTEMA) University of Mar del Plata and National Research Council (CONICET)  Mar del Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1gueda","family":"Massa","sequence":"additional","affiliation":[{"name":"National Institute of Fisheries Research and Development (INIDEP)  Mar del Plata Argentina"},{"name":"Marine and Coastal Research Institute (IIM y C) National Research Council (CONICET)  Mar del Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.07.043"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","DOI":"10.1007\/s12010-014-0823-4","article-title":"Purification and Characterization of Pepsin\u2010Solubilized Collagen From Skin of Sea Cucumber Holothuria Parva","author":"Adibzadeh N.","year":"2014","journal-title":"Applied Biochemistry and Biotechnology"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2020.100315"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.11.019"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010025\u201036684\u2010x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cosmetics4040039"},{"key":"e_1_2_7_8_1","unstructured":"AOAC International.1990. \u201cOfficial Methods of Analysis.\u201dAssociation of Official Analytical Chemists 16th ed. 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