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This study aimed to develop functional yogurt enriched with vitamins D\n                      <jats:sub>3<\/jats:sub>\n                      and B\n                      <jats:sub>12<\/jats:sub>\n                      by encapsulating these micronutrients in liposomal dispersions produced with food\u2010grade phospholipids, coated with pectin, and subjected to lyophilization. An optimal sucrose concentration was selected as a cryoprotectant to minimize detrimental effects of freeze\u2010drying on vesicle integrity. Lyophilized vesicles were characterized by bioactive retention and physicochemical properties. High retention of both vitamins was observed across most formulations, except those containing only hydrogenated saturated phospholipids, which showed reduced encapsulation efficiency. These vesicles were incorporated into yogurt, and the enriched product was evaluated for bioactive content, physicochemical and rheological properties, and sensory acceptability. The addition of vesicles did not negatively affect yogurt stability or consumer acceptance. In vitro digestion using the INFOGEST 2.0 protocol revealed increased vitamin D\n                      <jats:sub>3<\/jats:sub>\n                      bioaccessibility after the gastric phase only in samples containing Phospholipon 90G\u2010based liposomes; however, after the intestinal phase, all formulations showed enhanced availability. Vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      bioaccessibility remained consistent throughout digestion. Overall, results demonstrate a viable approach for coencapsulation and protection of hydrophobic and hydrophilic vitamins within a commonly consumed dairy product, offering a promising strategy for developing functional foods supporting daily nutritional supplementation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>\n                      Pectin\u2010coated and uncoated liposomes were successfully freeze\u2010dried with sucrose as a cryoprotectant. Purified phospholipids improved the bioaccessibility of vitamin D\n                      <jats:sub>3<\/jats:sub>\n                      after gastric digestion. Liposome enrichment did not alter yogurt stability or sensory acceptance. The strategy allows the co\u2010administration of hydrophilic and hydrophobic vitamins in dairy products.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70870","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T09:13:41Z","timestamp":1770369221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Yogurt Fortification With Lyophilized Liposomes Coencapsulating Vitamins D\n                    <sub>3<\/sub>\n                    and B\n                    <sub>12<\/sub>\n                    : Physicochemical Characterization, Sensory Evaluation and Static In Vitro Digestion"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-5872","authenticated-orcid":false,"given":"Let\u00edcia S.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Laboratory of Encapsulation and Functional Foods (LEnAlis), Department of Food Engineering, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduarda H.","family":"Luvizzotti","sequence":"additional","affiliation":[{"name":"Laboratory of Encapsulation and Functional Foods (LEnAlis), Department of Food Engineering, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marluci","family":"Ghiraldi","sequence":"additional","affiliation":[{"name":"Laboratory of Encapsulation and Functional Foods (LEnAlis), Department of Food Engineering, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus A.","family":"Chaves","sequence":"additional","affiliation":[{"name":"Laboratory of Encapsulation and Functional Foods (LEnAlis), Department of Food Engineering, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"},{"name":"Laboratory of Molecular Morphophysiology and Development (LMMD), Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha C.","family":"Pinho","sequence":"additional","affiliation":[{"name":"Laboratory of Encapsulation and Functional Foods (LEnAlis), Department of Food Engineering, School of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP)  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Official Methods of Analysis of AOAC International","author":"AOAC","year":"1990"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-022-05403-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2020.106225"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.01.025"},{"key":"e_1_2_8_7_1","unstructured":"BRASIL.2005.Minist\u00e9rio da Sa\u00fade. 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