{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T08:50:10Z","timestamp":1775897410321,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:italic>Synsepalum dulcificum<\/jats:italic>\n                      , commonly known as the miracle fruit, is a tropical plant noted for its unique biological activities. In this study, the anti\u2010influenza constituents from\n                      <jats:italic>S. dulcificum<\/jats:italic>\n                      leaves were identified via neuraminidase (NA) inhibition profiling. The ethanol extract was fractionated by polarity. The ethyl acetate (EA) fraction, which showed the most potent inhibition of bacterial NA (NAC) (IC\n                      <jats:sub>50<\/jats:sub>\n                      = 1.16 \u00b1 0.18\u00a0\u00b5g\/mL), was subjected to high\u2010resolution bioactivity profiling. A total of 172 sub\u2010fractions were assessed for NAC inhibitory activity. Active compounds were isolated based on their activity and identified using UPLC\u2010MS\/MS and NMR techniques. The inhibitory effects on both bacterial and viral neuraminidases (H3N2, H5N1 wild\u2010type\/mutant), as well as anti\u2010H1N1 activity, were evaluated. Molecular docking and dynamics studies were conducted to elucidate the binding mechanisms. Twelve compounds outperformed Oseltamivir against NAC, with galloylated flavonoid glycosides exhibiting high potency (IC\n                      <jats:sub>50<\/jats:sub>\n                      &lt; 10 \u00b5M). Notably, all compounds demonstrated efficacy against both wild\u2010type and Oseltamivir\u2010resistant (H274Y mutant) viral neuraminidases. Galloyl\u2010substituted flavonoid glycosides were identified as the principal active constituents. Although the isolated compounds exhibited only moderate inhibition of the H1N1 strain, the EA fraction showed stronger antiviral potency, with an EC\n                      <jats:sub>50<\/jats:sub>\n                      of 248.3 \u00b1 35.55\u00a0\u00b5g\/mL. Molecular dynamics simulations confirmed stable binding of the active scaffolds to NA. Therefore, this study provides a chemical and molecular mechanistic basis for considering\n                      <jats:italic>S. dulcificum<\/jats:italic>\n                      leaves as a potential edible resource worthy of further investigation for its anti\u2010influenza virus neuraminidase activity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70955","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:17:57Z","timestamp":1772993877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Resolution Neuraminidase Inhibition Profiling of\n                    <i>Synsepalum Dulcificum<\/i>\n                    Based on UPLC\u2010MS\/MS: A Study of Anti\u2010Influenza Constituents in a Potential Edible Resource"],"prefix":"10.1111","volume":"91","author":[{"given":"Haoyuan","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaming","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wensheng","family":"Xuan","sequence":"additional","affiliation":[{"name":"Guangdong Landfriend Biological Co., Ltd  Jiangmen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengchao","family":"Tu","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of the Ministry of Education (MOE) of China, College of Pharmacy Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Regenerative Medicine, Ministry of Education Jinan University  Guangzhou China"},{"name":"Department of Developmental &amp; Regenerative Biology Jinan University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arbres.2016.07.004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/emph\/eoy013"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14656566.2019.1626824"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2024.105980"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/children12010026"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.08.022"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118720"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7099"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a038398"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13185"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phyplu.2023.100417"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8948639"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115761"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153150"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2018.23.007"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.0253\u20102417.2014.01.021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/2221\u20101691.227999"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232012244"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2013.3084"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b02196"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116322"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2020.1830394"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728214.2022.2149734"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421\u2010020\u20100169\u20108"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130\u2010024\u201001152\u20103"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2018.18.006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112671"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.70955","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.70955","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.70955","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T07:53:56Z","timestamp":1775894036000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.70955"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,26]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/1750-3841.70955"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.70955","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,26]]},"assertion":[{"value":"2025-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70955"}}