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Using UPLC\u2010MS, GC\u2010IMS, and elemental profiling, we characterized how repeated extractions alter key tea constituents. Although flavonoids, polyphenols, polysaccharides, and amino acids remained detectable across all infusions, the concentrations of major bioactive compounds including gallic acid, its esters, and L\u2010theanine declined progressively from the first to the third infusion (GT1\u2013GT3), corresponding with reduced in vivo radical\u2010scavenging activity. Changes in volatile profiles and sensory evaluation further indicated noticeable shifts in flavor and taste with each infusion. Pearson correlation analysis revealed that most quantified compounds were positively associated with puckery, bitter, and acidic attributes, and negatively associated with sweetness and freshness. Variable Importance in Projection (VIP) analysis identified L\u2010lysine, L\u2010tyrosine, epicatechin, myricetin, theophylline, quercetin, and epigallocatechin as key contributors to sensory perception. These findings demonstrate that multiple infusions diversify flavor characteristics while modulating the bioactive profile of green tea, offering insights relevant to product development and consumer brewing practices.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71082","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T10:02:20Z","timestamp":1778061740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Three Successive Infusions: Scientific Insights Into a Traditional Green Tea Drinking Custom"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0055-7322","authenticated-orcid":false,"given":"Xiaoying","family":"Zhang","sequence":"first","affiliation":[{"name":"Chinese\u2010German Joint Institute for Natural Product Research, Shaanxi International Cooperation Demonstration Base Shaanxi University of Technology  Hanzhong 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Bindawa","family":"Isah","sequence":"additional","affiliation":[{"name":"Chinese\u2010German Joint Institute for Natural Product Research, Shaanxi International Cooperation Demonstration Base Shaanxi University of Technology  Hanzhong China"},{"name":"Department of Biochemistry Umaru Musa Yar'adua University  Katsina 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