{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:49:15Z","timestamp":1786121355305,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Piper longum<\/jats:italic>\n L., commonly consumed as a culinary spice and referred to as Piperis Fructus (PF) in traditional East Asian medicine, has been associated with various pharmacological activities; however, the anti\u2010osteoporotic potential of its ethanol extract has not been fully elucidated. In this study, we prepared a PF extract (containing 25.3\u00a0mg\/g piperine) and investigated its effects on osteoclastogenesis using RANKL\u2010stimulated RAW 264.7 cells (12.5\u201350\u00a0\u00b5g\/mL) and ovariectomized (OVX) rats orally administered PF (2.37 and 16.59\u00a0mg\/kg for 8 weeks). In vitro, anti\u2010osteoclastogenic effects were evaluated using TRAP, pit formation, F\u2010actin ring formation, western blot analysis, and qPCR, while in vivo effects were assessed using micro\u2010computed tomography and serum biomarker analysis. In vitro, PF significantly suppressed osteoclast formation (\n p<\/jats:italic>\n < 0.05\u20130.01), TRAP activity (\n p<\/jats:italic>\n < 0.01), resorption pit area (\n p<\/jats:italic>\n < 0.01), and F\u2010actin ring formation (\n p<\/jats:italic>\n < 0.05\u20130.01), accompanied by reduced c\u2010Fos (\n p<\/jats:italic>\n < 0.01) and NFATc1 protein expression (\n p<\/jats:italic>\n < 0.05\u20130.01), as well as the downregulation of osteoclast\u2010related genes (\n p<\/jats:italic>\n < 0.05\u20130.01). In OVX rats, PF\u2010H significantly reduced serum ALP (\n p<\/jats:italic>\n < 0.01) and showed a reduction in serum TRAP levels (\n p<\/jats:italic>\n < 0.05) and improved trabecular microarchitecture with increased BV\/TV (\n p<\/jats:italic>\n < 0.05), Tb.Th (\n p<\/jats:italic>\n < 0.01), Tb.N (\n p<\/jats:italic>\n < 0.01), and decreased Tb.Sp (\n p<\/jats:italic>\n < 0.05). Collectively, PF mitigates estrogen deficiency\u2013induced bone loss by suppressing osteoclast differentiation and function, suggesting its potential relevance for bone health; however, further studies are required.\n <\/jats:p>\n <\/jats:sec>\n \n Practical Applications<\/jats:title>\n \n Piper longum<\/jats:italic>\n L. is widely consumed as a culinary spice and represents a dietary source of bioactive phytochemicals. The present findings suggest that the ethanol extract of\n Piper longum<\/jats:italic>\n may help reduce bone loss by suppressing osteoclast activity. These results indicate its potential application as a functional food ingredient or nutraceutical for supporting bone health.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/1750-3841.71232","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T10:56:59Z","timestamp":1782298619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ethanol Extract of\n Piper longum<\/i>\n L., a Culinary Spice, Suppresses Osteoclastogenesis and Protects Against Ovariectomy\u2010Induced Bone Loss"],"prefix":"10.1111","volume":"91","author":[{"given":"Jin\u2010Ho","family":"Moon","sequence":"first","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Jeong","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seoung Jun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngwoo","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngjoo","family":"Sohn","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7256-9672","authenticated-orcid":false,"given":"Hyuk\u2010Sang","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01623458"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1241"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2019.01.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011\u20101282"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7939685"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14173667"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891\u2010022\u201005265\u20101"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9010017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.22889\/kjp.2023.54.3.137"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.1067"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2023.01.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10815"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.11005\/jbm.2014.21.4.233"},{"key":"e_1_2_10_15_1","volume-title":"Bonchohak","author":"Korean Oriental Medical Colleges","year":"2020"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph3051394"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010018\u20101381\u20102"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.797892"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20220307.201"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2021.110462"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/72855"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018\u2010021\u201002772\u20100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223\u2010013\u20109834\u20103"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1836"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c01667"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.081239"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050645"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.13422\/j.cnki.syfjx.20192149"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.12.018"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaw6143"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.71232","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T04:04:13Z","timestamp":1786075453000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.71232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/1750-3841.71232"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.71232","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71232"}}