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Its seeds, representing approximately 80%\u201395% of the fruit mass, constitute an abundant agro\u2010industrial byproduct with promising biotechnological potential. This study aimed to chemically characterize a 70% ethanolic extract obtained from\n                      <jats:italic>E. oleracea<\/jats:italic>\n                      seeds through liquid chromatography\u2013mass spectrometry (LC\u2013MS) annotation and total phenolic content (TPC) determination, as well as to investigate its antioxidant activity, prebiotic potential, cytotoxicity, and mutagenic safety profile. The extract was obtained by percolation using 70% ethanol. Antioxidant activity was evaluated by the DPPH assay, while prebiotic potential was assessed through submerged in vitro fermentation with\n                      <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                      LP90, monitoring bacterial growth and medium acidification. Cytotoxicity was evaluated in Caco\u20102 cells using a resazurin assay, and mutagenicity was investigated using the micronucleus and Ames tests. LC\u2013MS annotation revealed a phenolic\u2010rich profile, mainly composed of procyanidins. The extract showed a TPC of 123.12\u00a0\u00b1\u00a05.70\u00a0mg\u00a0GAE\/g dry weight and significant antioxidant activity (EC\n                      <jats:sub>50<\/jats:sub>\n                      \u00a0=\u00a011.79\u00a0\u00b1\u00a00.9\u00a0\u00b5g\/mL). Additionally, the extract promoted\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      growth and reduced medium pH, suggesting fermentable substrate potential. The extract also demonstrated low cytotoxicity (IC\n                      <jats:sub>50<\/jats:sub>\n                      \u00a0=\u00a0704.4\u00a0\u00b1\u00a041.8\u00a0\u00b5g\/mL) and no mutagenic effects. These findings support the valorization of\n                      <jats:italic>E. oleracea<\/jats:italic>\n                      seeds as a potential source of bioactive compounds for functional and nutraceutical applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71293","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:50:52Z","timestamp":1784029852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biological Safety and Functional Properties of A\u00e7a\u00ed Seed Extract: Antioxidant Capacity and Prebiotic Potential"],"prefix":"10.1111","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9398-2643","authenticated-orcid":false,"given":"Marcelo Pereira","family":"da Silva Junior","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda de Jesus Alves","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Chemistry Federal University of Maranh\u00e3o (UFMA)  S\u00e3o Lu\u00eds Maranh\u00e3o Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5401-4075","authenticated-orcid":false,"given":"Ana Laura","family":"Gagliardi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5066-5782","authenticated-orcid":false,"given":"Isabelle Guerreiro","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7356-9680","authenticated-orcid":false,"given":"Maria Eduarda Santos","family":"Camaceti","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Kawata","family":"Salga\u00e7o","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia","family":"Sivieri","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Health University of Araraquara (UNIARA)  Araraquara S\u00e3o Paulo 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