{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:16:40Z","timestamp":1791227800850,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Activation of intestinal bitter taste receptors can stimulate the release of glucagon\u2010like peptide\u20101 (GLP\u20101), thereby enhancing insulin secretion and exerting a hypoglycemic effect. In this study, a combination of bioactivity\u2010guided screening and chemical analysis was applied to search for potential components from\n                      <jats:italic>Momordica charantia<\/jats:italic>\n                      L. that exert hypoglycemic effects via bitter taste receptors. Combined with molecular networking and DeepSAT analysis, the active fraction was found to be rich in cucurbitane\u2010type triterpenoids, from which five pure compounds were isolated and identified. In conjunction with LC\u2010Q\u2010TOF\u2010MS\/MS analysis, an additional 24 cucurbitane\u2010type triterpenoids were also identified from the active fraction. Bioactivity evaluation revealed that five compounds exhibited stimulatory effects on GLP\u20101 secretion in Caco\u20102 cells; Compounds\n                      <jats:bold>2<\/jats:bold>\n                      and\n                      <jats:bold>4<\/jats:bold>\n                      increased GLP\u20101 secretion to 1.40\u2010fold and 1.35\u2010fold of the basal level at 5 \u00b5M, respectively. These results indicate that cucurbitane\u2010type triterpenoids may responsible for the potential hypoglycemic effect of\n                      <jats:italic>M. charantia<\/jats:italic>\n                      L. via bitter taste receptors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>Cucurbitane\u2010type triterpenoids derived from M. charantia L. are capable of stimulating the secretion of GLP\u20101 in intestinal cells, indicating their potential hypoglycemic activity. These compounds are promising as natural bioactive substances for the development of functional foods aimed at blood glucose homeostasis regulation. Furthermore, this provides a valuable research direction for the screening of natural hypoglycemic agents based on bitter taste receptors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71315","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T07:20:56Z","timestamp":1784791256000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive Bioactivity\u2010Guided Isolation, DeepSAT, and LC\u2010Q\u2010TOF\u2010MS\/MS Analysis to Reveal Hypoglycemic Constituents of\n                    <i>Momordica charantia<\/i>\n                    L"],"prefix":"10.1111","volume":"91","author":[{"given":"Hua\u2010Wei","family":"Lv","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science &amp; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals Zhejiang University of Technology  Hangzhou 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