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The strategy of employing multi\u2010strain probiotic combinations to achieve synergistic therapeutic effects is widely adopted in commercial applications. This study demonstrates that incorporating\n                      <jats:italic>Ligilactobacillus acidipiscis<\/jats:italic>\n                      YJ5 as a synergistic keystone strain into a base three\u2010strain probiotic consortium (CP3\u2010L) creates a potent low\u2010dose formulation (CP4\u2010L) with enhanced efficacy against loperamide\u2010induced constipation in mice. Although the CP3\u2010L consortium (\n                      <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                      SYS1 and T34,\n                      <jats:italic>Limosilactobacillus fermentum<\/jats:italic>\n                      W1) itself outperformed its constituent single strains, the CP4\u2010L consortium provided improvement in defecation parameters, intestinal barrier integrity, and inflammatory markers. It effectively restored interstitial cells of Cajal (ICC) markers (c\u2011Kit\/SCF) to enhance intestinal motility, downregulated colonic aquaporins to increase fecal water content, and selectively modulated the gut microbiota to raise beneficial\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      and fecal butyrate levels. These findings validate a mechanism\u2010guided approach to probiotic consortium design, where functional complementarity between strains yields synergistic, multi\u2010target anti\u2010constipation activity at a reduced dosage.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71332","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T09:46:55Z","timestamp":1787651215000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synergistic Addition of\n                    <i>Ligilactobacillus acidipiscis<\/i>\n                    YJ5 to a Probiotic Consortium for Enhanced Alleviation of Loperamide\u2010Induced Constipation in Mice"],"prefix":"10.1111","volume":"91","author":[{"given":"Fei","family":"Shen","sequence":"first","affiliation":[{"name":"College of Biological and Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou China"},{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2900-5901","authenticated-orcid":false,"given":"Congguo","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Biological and Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beingmate Co., Ltd  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Biological and Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunjing","family":"Han","sequence":"additional","affiliation":[{"name":"College of Biological and Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Du","sequence":"additional","affiliation":[{"name":"Hangzhou Kangyuan Food Science &amp; Technology Co., Ltd.  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqin","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghua","family":"He","sequence":"additional","affiliation":[{"name":"College of Biological and Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9206-3715","authenticated-orcid":false,"given":"Hao","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"College of Food Science and Technology Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3324\/yjbm.2016.89.3.389"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2022.100340"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.064"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.623698"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02873\u20100"},{"issue":"4","key":"e_1_2_8_7_1","article-title":"The Expression of Aquaporin 4 and 8 During DSS\u2010Induced Colitis","volume":"122","author":"Hardin J. 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