{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T23:51:56Z","timestamp":1793922716878,"version":"4.8.0"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201003434\u201024"],"award-info":[{"award-number":["APQ\u201003434\u201024"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>The INFOGEST 2.0 protocol is widely applied as a standardized static in vitro digestion model, and its use in studies involving plant\u2010based foods has increased substantially in recent years. This systematic review aimed to identify, classify, and critically evaluate methodological modifications applied to the INFOGEST 2.0 protocol in studies investigating plant\u2010based foods and supplements. A systematic search was conducted in the ScienceDirect, Scopus, and PubMed database, identifying 119 original research articles published between 2019 and 2024. Methodological modifications across the oral, gastric, and intestinal phases were extracted and analyzed according to the parameters altered and their underlying rationale. Approximately, half of studies (50.4%) deviated from standardized INFOGEST conditions, particularly regarding enzyme activity, pH, bile concentration, and digestion time. Importantly, these adaptations were not exclusively driven by the plant\u2010based nature of the food matrices, but also reflected methodological factors related to the study population or objectives, including aging\u2010related physiological conditions, specific experimental needs, the tracing of particular compounds (e.g., minerals and phenolic compounds), and blank subtraction. While such modifications may enhance physiological relevance or analytical accuracy, they introduce a trade\u2010off with interstudy comparability, highlighting the need for clearer reporting practices and consensus\u2010based extensions of the INFOGEST framework to balance physiological relevance with methodological consistency.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71356","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:18:34Z","timestamp":1787311114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Methodological Modifications of the INFOGEST 2.0 Protocol in Plant\u2010Based Food Studies: A Systematic Review"],"prefix":"10.1111","volume":"91","author":[{"given":"Matheus Braga","family":"Mendes","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Federal University of Juiz de Fora  Juiz de Fora Minas Gerais 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