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Microbanks preserved\n                      <jats:italic>Lactococcus lactis<\/jats:italic>\n                      and\n                      <jats:italic>Levilactobacillus brevis<\/jats:italic>\n                      for 1 year, whereas\n                      <jats:italic>Pediococcus<\/jats:italic>\n                      spp. and\n                      <jats:italic>Lactiplantibacillus pentosus<\/jats:italic>\n                      and\n                      <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                      were viable for 22 months. The preserved LAB were resuscitated in cucumber, carrot, or sweet potato juice after 24\u201330\u00a0h of incubation at ambient temperature. Cucumber fermentation medium (CFM), used for the pre\u2010adaptation of the starter cultures to 342\u00a0mM (2%) sodium chloride (NaCl), sustained the growth of the preserved LAB to maximum cell densities in 12\u201324\u00a0h. Pre\u2010adaptation of cultures in 100\u00a0mL CFM supported quick acid production, but inoculation of up to 10\u00a0L of CFM with a 10\u00a0\u00b5L loop full of cells sufficed for a complete fermentation and biomass production. Cultures of\n                      <jats:italic>Lc. lactis<\/jats:italic>\n                      and\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      reached maximum cell densities in CFM in 12\u00a0h, whereas\n                      <jats:italic>L. pentosus<\/jats:italic>\n                      presented a 2\u00a0h delay (14\u00a0h).\n                      <jats:italic>Lev. brevis<\/jats:italic>\n                      and\n                      <jats:italic>Pediococcus<\/jats:italic>\n                      spp. needed 16\u00a0h incubation to achieve maximum cell densities in CFM. Acid production by the LAB revived in CFM supplemented with 342\u00a0mM NaCl, 18\u00a0mM calcium chloride, and 18\u00a0mM calcium hydroxide reduced the pH to 5.60\u00a0\u00b1\u00a00.51 by the time they reached maximum cell densities. These observations led to a method for the maintenance of LAB in Microbanks in a household freezer, and for their use in preparing starter cultures for commercial cucumber fermentations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>Implementation of commercial cucumber fermentations with reduced salt demands the utilization of starter cultures to guide fermentations to the endpoint, assure acid production, and prevent processing defects. The methods described here create opportunities for processors to generate starter cultures in\u2010house for commercial\u2010scale cucumber fermentations using functional strains isolated from such habitats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71363","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T03:27:56Z","timestamp":1787369276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Methods for Maintaining and Using Lactic Acid Bacteria Starter Cultures for Commercial Cucumber Fermentation Brined With Low Salt"],"prefix":"10.1111","volume":"91","author":[{"given":"Amanda B.","family":"Santos","sequence":"first","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clinton A.","family":"Page","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Genomics University of North Carolina  Charlotte North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3506-7796","authenticated-orcid":false,"given":"Ilenys M.","family":"P\u00e9rez\u2010D\u00edaz","sequence":"additional","affiliation":[{"name":"US Department of Agriculture (USDA), Agricultural Research Service (ARS), Food Science and Market Quality and Handling Research Unit  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda B. O.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco No\u00e9","family":"Arroyo\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Food Biotechnology Instituto de la Grasa (IG\u2010CSIC)  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2012.10.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.70490"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1257\u20101259.2004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.1978.tb00804.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102621.1988.tb10192.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.16084"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/fmic.1993.1054"},{"issue":"1","key":"e_1_2_10_9_1","first-page":"19","article-title":"Storage and Repeated Recovery of Neisseria gonorrhoeae Using Cryovials","volume":"52","author":"Moyes A.","year":"1995","journal-title":"British Journal of Biomedical Science"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12132455"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.11.025"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750\u20103841.2008.00795.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.13107"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.16345"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.272"},{"issue":"6","key":"e_1_2_10_16_1","first-page":"215","article-title":"Efficiency of Microbank\u00ae Systems for the Conservation of Microorganisms Relevant to Veterinary Medicine and Others Which Are Not Easy to Cultivate","volume":"108","author":"Seidel K. 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