{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:17:43Z","timestamp":1787980663297,"version":"build-2784847793"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Dairy peptides and plant polyphenols attract significant attention for their bioactive properties; however, the synergistic antioxidant mechanism of buttermilk peptides and myricetin remains unclear. In this study, buttermilk proteins from Lacprodan MFGM\u201010, a milk fat globule membrane (MFGM)\u2010enriched buttermilk powder, were subjected to multi\u2010enzyme hydrolysis using papain (Pap), alkaline protease (AP), and neutral protease (NP) at an enzyme concentration of 6000 U\/g in different combinations. The results showed that the synergistic application of enzymes significantly increased the degree of hydrolysis (DH), with the AP group reaching a peak DH of approximately 42% at 150\u00a0min. Two candidate peptides, WGSPP and SWPWQ, were selected from the screened buttermilk peptide fractions. Through Caco\u20102 transmembrane transport validation and molecular docking, it is confirmed that these peptides can non\u2010competitively bind to acetylcholinesterase (AChE) via molecular docking, with binding energies of \u221212.7 and \u221211.1\u00a0kcal\/mol, respectively. Additionally, myricetin exhibits a strong binding energy, with a binding energy of \u22129.5\u00a0kcal\/mol. In an L6 myoblast injury model induced by rotenone, combined treatment significantly enhances AChE inhibitory rate, reduces reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restores superoxide dismutase (SOD) activity and the NAD\n                      <jats:sup>+<\/jats:sup>\n                      \/NADH ratio, and improves mitochondrial membrane potential and ATP levels, without demonstrating cytotoxicity. Buttermilk\u2010derived peptides and myricetin exert synergistic antioxidant effects by improving redox homeostasis and mitochondrial function, thereby alleviating oxidative stress\u2010induced injury in rotenone\u2010induced L6 cells. These findings provide preliminary evidence for the potential role of these compounds in oxidative stress\u2010associated skeletal muscle health; however, direct sarcopenia\u2010related endpoints were not evaluated in the present study.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71381","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T03:20:36Z","timestamp":1787973636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Enzyme Hydrolysis and Ultrafiltration\u2010Based Optimization, Identification, and Characterization of Buttermilk Peptides and Myricetin for Enhancing Antioxidant Synergy"],"prefix":"10.1111","volume":"91","author":[{"given":"Wenjing","family":"Niu","sequence":"first","affiliation":[{"name":"School of Life Sciences Northeast Forestry University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Harbin Institute of Technology  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Life Sciences Northeast Forestry University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yejun","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Life Sciences Northeast Forestry University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Xiao","sequence":"additional","affiliation":[{"name":"Inner Mongolia Mengniu Dairy (Group) Co., Ltd.  Nei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Niu","sequence":"additional","affiliation":[{"name":"Inner Mongolia Mengniu Dairy (Group) Co., Ltd.  Nei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Harbin Institute of Technology  Harbin China"},{"name":"Ausnutria Dairy (China) Co. Ltd.  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