{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:56:13Z","timestamp":1790567773858,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      This study evaluated the aqueous extract of\n                      <jats:italic>Pimpinella anisum<\/jats:italic>\n                      seeds (AE\u2010PAS) and its green\u2010synthesized silver nanoparticles (PA\u2010AgNPs) using integrated in vitro, in vivo, and in silico approaches. LC\u2013MS analysis identified 26 phytoconstituents, mainly sinapic acid (15.78%), hispidulin (15.35%), caffeic acid (11.09%), and rutin (8.86%). Total phenolic and flavonoid contents were 45.2\u00a0\u00b1\u00a02.1\u00a0mg\u00a0GAE\/g DE and 28.7\u00a0\u00b1\u00a01.5\u00a0mg\u00a0QE\/g DE, respectively. UV\u2013Vis spectroscopy, scanning electron microscopy (SEM), Fourier\u2010transform infrared (FTIR) spectroscopy, and X\u2010ray diffraction (XRD) supported nanoparticle formation, indicating dry\u2010state particle or agglomerate features of approximately 60\u201390 nm, a surface plasmon resonance (SPR) peak at 421 nm, 50.81% crystallinity, and a crystallite\u2010domain size of 22.57 \u00b1 1.2 nm. AE\u2010PAS showed measurable antioxidant and albumin\u2010denaturation\u2010inhibitory responses (DPPH IC\n                      <jats:sub>50<\/jats:sub>\n                      \u00a0=\u00a0130.63\u00a0\u00b1\u00a01.3\u00a0\u00b5g\/mL; albumin\u2010denaturation IC\n                      <jats:sub>50<\/jats:sub>\n                      \u00a0=\u00a0721.79\u00a0\u00b1\u00a01.7\u00a0\u00b5g\/mL). PA\u2010AgNPs produced measurable inhibition zones in agar well diffusion screening, but antibacterial potency could not be established because MIC and MBC were not determined. The PA\u2010AgNP DPPH result lacked a nanoparticle\u2010only blank and should be regarded as an apparent assay\u2010specific estimate. Hemolysis was concentration dependent, increasing from 5.20% at 50\u00a0\u00b5g\/mL to 29.93% at 100\u00a0\u00b5g\/mL. In behavioral models, PA\u2010AgNPs (0.1\u00a0mg\/kg) produced measurable antidepressant\u2010like and analgesic responses; these findings represent preliminary behavioral evidence rather than confirmed efficacy. Molecular docking predicted favorable binding scores (\u22128.0 to \u22129.6\u00a0kcal\/mol) for selected extract phytochemicals against MAO\u2010A\/B, COX\u20101\/2, and TNF\u2010\u03b1 but did not establish target inhibition or a biological mechanism. Overall, the findings are preliminary and require comprehensive physicochemical characterization, appropriate silver\u2010ion and material controls, quantitative antimicrobial testing, pharmacokinetic and mechanistic validation, and expanded safety assessment before biomedical translation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71388","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:12:57Z","timestamp":1788232377000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biogenic Silver Nanoparticles Mediated by Phytoconstituents of the Edible Spice\n                    <i>Pimpinella anisum<\/i>\n                    : Characterization and In Vitro, In Vivo, and In Silico Biological Evaluation"],"prefix":"10.1111","volume":"91","author":[{"given":"Ibtissam","family":"Laib","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Biology El Oued University  El Oued Algeria"},{"name":"Laboratory of Biodiversity and Biotechnology Applications in Agriculture University of El Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis Ben","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology El Oued University  El Oued Algeria"},{"name":"Laboratory of Biology, Environment and Health, Faculty of Natural and Life Sciences University of El Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soundes","family":"Guedda","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology El Oued University  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nardjes","family":"Guedda","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology El Oued University  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naglaa A.","family":"Abd\u2010Elkarim","sequence":"additional","affiliation":[{"name":"Department of Food and Dairy Sciences, Faculty of Agriculture Qena University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef","family":"Benaissa","sequence":"additional","affiliation":[{"name":"VPRS Laboratory, Chemistry Department, Faculty of Mathematics and Matter Sciences University of KASDI Merbah  Ouargla Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nariman","family":"Essmat","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elhafnaoui","family":"Lanez","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology El Oued University  El Oued Algeria"},{"name":"Faculty of Exact Sciences, Department of Chemistry, VTRS Laboratory University of El Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed F.","family":"Abouelenein","sequence":"additional","affiliation":[{"name":"Department of Insurance and Risk Management, College of Business Imam Mohammad Ibn Saud Islamic University (IMSIU)  Riyadh, Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2788-7839","authenticated-orcid":false,"given":"Ahmed I.","family":"Osman","sequence":"additional","affiliation":[{"name":"School of Sciences, Psychology, Arts and Humanities, Computing, Engineering, and Sport Canterbury Christ Church University  Canterbury UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Mahfouz","sequence":"additional","affiliation":[{"name":"Medicinal and Aromatic Plants Department Desert Research Center  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9318-5256","authenticated-orcid":false,"given":"Ahmed K.","family":"Rashwan","sequence":"additional","affiliation":[{"name":"Department of Food and Dairy Sciences, Faculty of Agriculture Qena University  Qena Egypt"},{"name":"Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568\u2010023\u201001619\u20107"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092901"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S113193"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbt.2024.12.003"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/gps\u20102020\u20100051"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1485525"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/aoc.70251"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2015.11.005"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3MA00089C"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2298\/APT2455001B"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145711001842"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.06.017"},{"issue":"59","key":"e_1_2_11_14_1","article-title":"The Tail Suspension Test","volume":"00","author":"Can A.","year":"2012","journal-title":"JoVE"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc4010004"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201082349\u2010z"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hybadv.2025.100389"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.2478\/AMB\u20102024\u20100060"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00284"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rechem.2023.101108"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13204\u2010024\u201003076\u20105"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312706"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/138945011793591626"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10081573"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201072356\u20105"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2024.138800"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26164986"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/24701556.2024.2358338"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/17518253.2024.2412601"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nxmate.2025.101275"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.118"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202505506"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202502270"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20100500\u20106\u2010121"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44174\u2010025\u201000295\u20104"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.107262"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010012\u20100837\u2010z"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2024.1508707"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2025.102071"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2013.3925"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.70681"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2021.105290"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/24701556.2024.2354504"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma15020427"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr10081521"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2015.1124808"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184331"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.03.050"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/2228\u20105326\u20102\u201032"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0502698"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/17458080.2015.1077534"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12123"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100039\u20101688491"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rechem.2022.100663"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.044"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1080\/23312025.2019.1673688"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA04164H"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201027171\u20101"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12071436"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12668\u2010023\u201001125\u2010x"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814774-0.00002-5"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jallcom.2021.162502"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25133022"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfa.2019.124167"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2024.112265"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71388","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.71388","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71388","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:21:09Z","timestamp":1790565669000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.71388"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":64,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/1750-3841.71388"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.71388","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2026-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71388"}}