{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:56:52Z","timestamp":1790567812449,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Foodborne diseases and microbial spoilage remain major threats to food safety and shelf life. This study systematically evaluated\n                      <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                      C\u201035 for antimicrobial activity, mechanisms, genomic safety, metabolite profile, and application in bread. Among 27 lactic acid bacterial strains,\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      C\u201035 showed strong broad\u2010spectrum inhibition against\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      ,\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ,\n                      <jats:italic>Aspergillus niger<\/jats:italic>\n                      , and\n                      <jats:italic>Penicillium solitum<\/jats:italic>\n                      , significantly outperforming\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      ATCC 8014 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Neutralization reduced residual activity to approximately 35%\u201355%, whereas protease\u2010treated cell\u2010free supernatant generally retained more than 60% activity, indicating that organic acids were the major antimicrobial contributors and proteinaceous substances played a secondary role. Scanning electron microscopy (SEM) revealed cell shrinkage, membrane disruption, spore collapse, and surface deformation in treated microorganisms. Whole\u2010genome sequencing identified a 2.95\u00a0Mb genome with 2803 protein\u2010coding genes and four predicted secondary metabolite biosynthetic gene clusters, whereas no antibiotic resistance or virulence\u2010associated genes meeting the screening criteria were detected. LC\u2013MS metabolomics identified 14 antimicrobial\u2010associated compounds and 416 significantly differential metabolites between\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      C\u201035 and\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      ATCC 8014, further supporting its enhanced bioactivity. Luteolin, mangiferin, indole\u20103\u2010lactic acid, glutaric acid, and naringenin were enriched in\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      C\u201035 and may contribute synergistically to its antimicrobial effects. In bread, visible mold appeared on Days 6, 8, and 10 in the control,\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      ATCC 8014, and\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      C\u201035 groups, respectively, demonstrating its practical preservation potential. Together, these findings indicate that the strain\u2010specific genomic and metabolomic features of\n                      <jats:italic>L. plantarum<\/jats:italic>\n                      C\u201035 are associated with its strong antimicrobial performance and support its potential application as a clean\u2010label biopreservative in bread systems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71392","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:47:12Z","timestamp":1790156832000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating Genomics and Metabolomics to Identify Lactic Acid Bacteria With Broad\u2010Spectrum Antimicrobial Activity and Validation in a Bread 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