{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:57:27Z","timestamp":1790517447095,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Cultured meat, produced through the cultivation of animal cells, represents a sustainable and ethical alternative to traditional livestock farming. The cultivation and expansion of animal cells for cultured meat production rely heavily on extracellular matrix (ECM) proteins, which are needed for cell adhesion, proliferation, and differentiation. However, common ECM components such as gelatin, collagen, vitronectin, and Matrigel are mainly derived from animal sources, raising ethical concerns and sustainability issues. In this study, we used in silico analysis to identify edible plant\u2010derived proteins containing Arg\u2013Gly\u2013Asp (RGD) motifs, which can promote cell adhesion and proliferation like ECM proteins. Peanut (\n                      <jats:italic>Arachis hypogaea<\/jats:italic>\n                      ) and quinoa (\n                      <jats:italic>Chenopodium quinoa<\/jats:italic>\n                      ) were identified as promising candidates. Water\u2010soluble extracts were prepared from the plant seeds and evaluated for their ability to support the adhesion, proliferation, and differentiation of murine C2C12 and immortalized bovine satellite cells. We found that the extracts predominantly contained low\u2010molecular\u2010weight proteins, and cell culture surfaces coated with either extract supported cell attachment and proliferation at levels comparable to those achieved with animal\u2010derived ECM proteins. Analysis of the most abundant proteins in each plant extract as determined by mass spectrometry confirmed the presence of RGD motif\u2010containing proteins. Subsequently, one RGD\u2010containing protein identified from each extract was produced using a cell\u2010free expression system, and lysates containing the produced proteins were evaluated for their ability to support cell attachment and proliferation. The results indicated enhanced cell attachment and proliferation, indicating a potential role of the proteins. Our findings demonstrate that peanut and quinoa protein extracts are promising and inexpensive animal\u2010free alternatives for surface functionalization in cultured meat applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71509","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:45:06Z","timestamp":1790516706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quinoa and Peanut Extracts as Alternatives to Animal\u2010Derived Extracellular Matrix Proteins in Cultured Meat Production"],"prefix":"10.1111","volume":"91","author":[{"given":"Dominik","family":"Mundt","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo\u2010Hui","family":"Chiu","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schillberg","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME  Aachen Germany"},{"name":"Institute for Molecular Biotechnology RWTH Aachen University  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1137-1822","authenticated-orcid":false,"given":"Che Julius","family":"Ngwa","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology IME  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2023.114118","article-title":"Allergenicity Evaluation of Quinoa Proteins\u2014A Study in Brown Norway Rats","volume":"182","author":"Ballegaard A.\u2010S. 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X.Zhu S.Chen M.Li X.Liu andY.Pan.2014. \u201cTrend Analysis for Blue and Green Water Resources in the Weihe River Basin of Northwest China During the Past Thirty Years and in the Near Future.\u201d In2014 The Third International Conference on Agro\u2010Geoinformatics.IEEE.1\u20135.","DOI":"10.1109\/Agro-Geoinformatics.2014.6910620"},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2023.1260856","article-title":"Identification and Characterization of the cupin_1 Domain\u2010Containing Proteins in Ma Bamboo (Dendrocalamus latiflorus) and Their Potential Role in Rhizome Sprouting","volume":"14","author":"Zhu P.\u2010K.","year":"2023","journal-title":"Frontiers in Plant Science"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71509","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.71509","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71509","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:45:12Z","timestamp":1790516712000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.71509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,27]]},"references-count":43,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/1750-3841.71509"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.71509","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,27]]},"assertion":[{"value":"2026-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71509"}}