{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T06:33:34Z","timestamp":1793082814412,"version":"4.5.0"},"reference-count":66,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The wild edible fruit\n                      <jats:italic>Ficus hispida<\/jats:italic>\n                      Linn (\n                      <jats:italic>F. hispida<\/jats:italic>\n                      ), traditionally used for diabetes management, remains unexplored with respect to bioactive constituents and underlying mechanisms. Therefore, this study investigated the antidiabetic potential of\n                      <jats:italic>F. hispida<\/jats:italic>\n                      fruits through comprehensive\u00a0phyto\u2010metabolomic\u00a0profiling integrated with biochemical assays and in silico approaches. Fruit extracts were prepared via cold maceration and evaluated for total phenolic and flavonoid contents, antioxidant activity, and inhibition of \u03b1\u2010amylase and \u03b1\u2010glucosidase. Comprehensive untargeted metabolite profiling was performed using GC\u2013QqQ\u2013MS\/MS and LC\u2013ESI\u2013Orbitrap\u2013MS\/MS, while targeted analysis was conducted by HPLC. Metabolite annotation and data processing were conducted using Compound Discoverer (v3.2.2.31) and NIST libraries, followed by PCA, PLS\u2010DA, and hierarchical clustering using MetaboAnalyst 6.0. Methanol and hydroalcoholic extracts exhibited the highest phenolic (78.85\u00a0\u00b1\u00a00.58 and 74.68\u00a0\u00b1\u00a00.24\u00a0mg GAE\/g) and flavonoid contents (89.03\u00a0\u00b1\u00a00.45 and 71.91\u00a0\u00b1\u00a01.96\u00a0mg NE\/g, respectively), which corresponded with their stronger \u03b1\u2010amylase inhibition (IC\n                      <jats:sub>50<\/jats:sub>\n                      : 91.11\u00a0\u00b1\u00a03.54 and 75.96\u00a0\u00b1\u00a03.48\u00a0\u00b5g\/mL) and \u03b1\u2010glucosidase inhibition (IC\n                      <jats:sub>50<\/jats:sub>\n                      : 101.44\u00a0\u00b1\u00a02.04 and 89.29\u00a0\u00b1\u00a03.03\u00a0\u00b5g\/mL, respectively). GC\u2012MS\/MS and LC\u2012MS\/MS profiling revealed 160 and 759 putatively annotated metabolites (MSI 2), respectively. Furthermore, in silico molecular docking identified new \u03b1\u2010amylase and \u03b1\u2010glucosidase inhibitors, that is, desmodol (\u22128.324\u00a0kcal\/mol) and demethylbatatasin IV (\u22127.062\u00a0kcal\/mol), respectively. Complementarily, molecular dynamics simulation demonstrated the stable binding of both desmodol\u2013\u03b1\u2010amylase and demethylbatastasin IV\u2013\u03b1\u2010glucosidase complexes, supporting their potential therapeutic relevance as antidiabetic phytoconstituents. Overall, this study highlights the potential of integrated phyto\u2010metabolomics and chemometrics for identifying antidiabetic markers with applications in functional foods and therapeutics.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71518","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:33:27Z","timestamp":1791556407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phyto\u2010Metabolomic Insights Into\n                    <i>Ficus hispida<\/i>\n                    Linn.: Antioxidant Activity, Polyphenolic Content, and Antidiabetic Marker Discovery via LC\u2013ESI\u2013Orbitrap\u2013MS\/MS and GC\u2013QqQ\u2013MS\/MS"],"prefix":"10.1111","volume":"91","author":[{"given":"Kotla","family":"Kotesh","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Hajipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efficiency","family":"Myrsing","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Hajipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tejaswini Chandrakant","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Hajipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangita Indal","family":"Gadilohar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Hajipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4850-6783","authenticated-orcid":false,"given":"P.","family":"Ramalingam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Hajipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2017.12.011"},{"key":"e_1_2_17_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.10.035"},{"key":"e_1_2_17_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386454-3.01219-7"},{"key":"e_1_2_17_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2023.e01593"},{"key":"e_1_2_17_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2018.05.010"},{"key":"e_1_2_17_7_1","unstructured":"BRDC. n.d.Wild Edible Fruits of Meghalaya.Bio Resources Development Centre."},{"key":"e_1_2_17_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz382"},{"issue":"4","key":"e_1_2_17_9_1","first-page":"334","article-title":"Medicinal and Nutritional Aspects of Wild Edible Fruits From Western Ghat of India","volume":"16","author":"Dharmappa K. K.","year":"2022","journal-title":"International Journal of Green Pharmacy"},{"key":"e_1_2_17_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2018.07.003"},{"key":"e_1_2_17_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/life10120349"},{"key":"e_1_2_17_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27072244"},{"key":"e_1_2_17_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116239"},{"key":"e_1_2_17_14_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70591"},{"key":"e_1_2_17_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2016.2834"},{"key":"e_1_2_17_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2008.07.004"},{"key":"e_1_2_17_17_1","unstructured":"Hazarika T. K. andS.Marak.2015. \u201cWild Edible Fruits of Meghalaya North\u2010East India: an Unexplored Potential for Nutritional Security and Economic Prosperity.\u201d III International Symposium on Underutilized Plant Species 1241."},{"key":"e_1_2_17_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7390359"},{"key":"e_1_2_17_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.11.071"},{"key":"e_1_2_17_20_1","unstructured":"ICH Harmonised Tripartite Guideline.2005.Validation of Analytical Procedures: Text and Methodology. Q2 (R1)."},{"key":"e_1_2_17_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13659\u2010020\u201000233\u20105"},{"key":"e_1_2_17_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2024.12536"},{"key":"e_1_2_17_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf990146l"},{"issue":"6","key":"e_1_2_17_24_1","first-page":"209","article-title":"Ethno\u2010Medicinal Species of Genus Ficus L. Used to Treat Diabetes in Pakistan","volume":"1","author":"Khan Y.","year":"2011","journal-title":"Journal of Applied Pharmaceutical Science"},{"key":"e_1_2_17_25_1","first-page":"6","article-title":"Ethnomedicinal Uses of Genus Ficus L. in Pakistan","volume":"1","author":"Khan Y.","year":"2011","journal-title":"International Poster Journal of Science and Technology"},{"key":"e_1_2_17_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2026.118991"},{"key":"e_1_2_17_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01727.x"},{"key":"e_1_2_17_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac103245b"},{"key":"e_1_2_17_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2016.07.020"},{"key":"e_1_2_17_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.136113"},{"key":"e_1_2_17_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11071228"},{"key":"e_1_2_17_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20192133"},{"key":"e_1_2_17_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-010-0102-2"},{"key":"e_1_2_17_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0367\u2010326x(02)00225\u20103"},{"key":"e_1_2_17_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1573(200009)14:6<457::AID-PTR610>3.0.CO;2-8"},{"issue":"1","key":"e_1_2_17_36_1","first-page":"96","article-title":"Plant Wealth of Northeast India With Reference to Ethnobotany","volume":"8","author":"Mao A. A.","year":"2009","journal-title":"Indian Journal of Traditional Knowledge"},{"key":"e_1_2_17_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-31885-7"},{"key":"e_1_2_17_38_1","volume-title":"Indian Materia Medica","author":"Nadkarni K. M.","year":"1976"},{"key":"e_1_2_17_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942910903203164"},{"key":"e_1_2_17_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132244"},{"key":"e_1_2_17_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272\u2010013\u20100069\u20107"},{"key":"e_1_2_17_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-7248-3_27"},{"key":"e_1_2_17_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae253"},{"key":"e_1_2_17_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.05.088"},{"key":"e_1_2_17_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2019.04.016"},{"key":"e_1_2_17_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9904509"},{"key":"e_1_2_17_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184331"},{"key":"e_1_2_17_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2022.111874"},{"key":"e_1_2_17_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.694362"},{"key":"e_1_2_17_50_1","first-page":"255","article-title":"In Vitro Antineoplastic Effect of Ficus hispida L. Plant Against Breast Cancer Cell Lines","volume":"3","author":"Pratumvinit B.","year":"2009","journal-title":"Journal of Medicinal Plants Research"},{"key":"e_1_2_17_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476\u2010511x\u201011\u201098"},{"key":"e_1_2_17_52_1","doi-asserted-by":"publisher","DOI":"10.3923\/rjb.2011.58.67"},{"key":"e_1_2_17_53_1","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v9i2.16"},{"key":"e_1_2_17_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010022\u201023337\u20106"},{"issue":"8","key":"e_1_2_17_55_1","first-page":"241","article-title":"Traditional Medicinal Knowledge of Underutilized Minor Fruits as Medicine in Manipur","volume":"4","author":"Singh S. R.","year":"2014","journal-title":"International Journal of Agricultural Science"},{"key":"e_1_2_17_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-32-9174-4_5"},{"key":"e_1_2_17_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.06.042"},{"key":"e_1_2_17_58_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015821527621"},{"key":"e_1_2_17_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112257"},{"key":"e_1_2_17_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.08.041"},{"key":"e_1_2_17_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237248"},{"key":"e_1_2_17_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.145468"},{"key":"e_1_2_17_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2024.104227"},{"key":"e_1_2_17_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1730-9"},{"key":"e_1_2_17_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2018.07.004"},{"key":"e_1_2_17_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2016.02.002"},{"key":"e_1_2_17_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.11.016"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71518","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.71518","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.71518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T05:46:56Z","timestamp":1793080016000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.71518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":66,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/1750-3841.71518"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.71518","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71518"}}