{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T06:33:24Z","timestamp":1793082804450,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:italic>Malus micromalus<\/jats:italic>\n                      Makino, a fruit crop with a millennium\u2010long cultivation heritage in China, has hitherto received limited systematic investigation regarding its bioactive principles and the mechanistic underpinnings of its glucose\u2010modulating effects. Sixteen compounds were isolated and structurally identified from the fruit. The bioactive components and action modes were systematically explored via network pharmacology, in vitro bioassays, enzyme kinetics, molecular simulation, and in vivo validation. ESR1 and MAPK3 were predicted as potential hub targets involved in insulin resistance and oxidative stress regulation. Oleanolic acid, ursolic acid, and hyperoside exhibited dual functional activities, with potent \u03b1\u2010glucosidase inhibitory effects (IC\n                      <jats:sub>50<\/jats:sub>\n                      = 0.037, 0.038, and 0.047\u00a0mM, respectively) stronger than acarbose in this yeast\u2010derived enzyme assay system, plus remarkable DPPH, and ABTS\n                      <jats:sup>+<\/jats:sup>\n                      radical scavenging capacities. Enzyme kinetic analysis demonstrated a noncompetitive inhibition mode, with unchanged\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>m<\/jats:sub>\n                      value, and dose\u2010dependently decreased\n                      <jats:italic>V<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      . Molecular docking indicated that OA and UA had high binding affinities with ESR1 and MAPK3. Notably, 100\u2010ns molecular dynamics simulation suggested that OA could form sustained hydrogen bonds with ESR1 and maintain the structural stability of the receptor\u2013ligand complex. Within the in vivo setting, OA treatment at 25 and 50\u00a0mg\/kg engendered dose\u2010dependent improvements in systemic glucose handling, peripheral insulin sensitivity, and circulating lipid profiles among HFD\u2010induced obese animals. Collectively, this study clarifies the bioactive constituents and metabolic regulatory effects of\n                      <jats:italic>M. micromalus<\/jats:italic>\n                      Makino, and provides scientific evidence for its development as a functional food ingredient for glycemic management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>\n                      The present findings offer direct translational value for the functional food sector. The potent \u03b1\u2010glucosidase inhibitory activity of oleanolic acid (OA, IC\n                      <jats:sub>50<\/jats:sub>\n                      = 0.037\u00a0mM)\u2014surpassing acarbose by approximately sevenfold\u2014positions\n                      <jats:italic>M. micromalus<\/jats:italic>\n                      fruit extracts as promising candidates for glycemic\u2010management food ingredients. Given its noncompetitive inhibition kinetics, OA retains efficacy irrespective of carbohydrate load, a critical advantage for incorporation into staple foods with variable starch content. From a formulation standpoint, the ethyl acetate fraction enriched in triterpenoids and flavonoids could be developed as standardized extracts for functional beverages, bakery products, or nutraceutical capsules. The established industrial processing of\n                      <jats:italic>M. micromalus<\/jats:italic>\n                      into fruit wine and preserves further supports scalable valorization. However, the poor aqueous solubility of pentacyclic triterpenoids necessitates future work on delivery systems (e.g., nano\u2010emulsions or liposomal encapsulation) to enhance bioavailability and thermal stability during food processing. In addition, the dual antioxidant\u2013hypoglycemic profile of these constituents suggests potential as multifunctional natural preservatives that simultaneously extend shelf life and confer metabolic health benefits. Subsequent studies should evaluate the sensory impact, storage stability, and consumer acceptability of\n                      <jats:italic>M. micromalus<\/jats:italic>\n                      \u2010fortified food prototypes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.71532","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:23:11Z","timestamp":1790842991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Hypoglycemic and Antioxidant Components From\n                    <i>Malus micromalus<\/i>\n                    Makino Fruits With Potential for Functional Food Applications"],"prefix":"10.1111","volume":"91","author":[{"given":"Baocong","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Engineering, College of Chemical Engineering Northwest University  Xi'an 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