{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:03:36Z","timestamp":1762340616747},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T00:00:00Z","timestamp":1369699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The study aimed to investigate the relationship between gastroduodenal disease and the diversity of the cagA 3\u2032 variable region and the amino acid polymorphisms in the Glu\u2010Pro\u2010Ile\u2010Tyr\u2010Ala (EPIYA) segments of the CagA C\u2010terminal region of <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>elicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gastric mucosal specimens from 170 patients in our center (Nanjing, Jiangsu Province, China) were collected and the genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> of the <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u2009pylori<\/jats:italic> strains was extracted directly from biopsied specimens. Polymerase chain reaction (PCR) was used to amplify the <jats:italic>cag<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content><\/jats:italic> gene, and diversity in its 3\u2032 variable region was assessed by direct sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 154 (90.6%) <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u2009pylori<\/jats:italic> isolates were <jats:italic>cag<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content><\/jats:italic>\u2010positive, but the presence of this gene alone was not associated with the type of gastroduodenal disease. A total of 151 (88.8%) strains had the <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>ast <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>sian type <jats:styled-content style=\"fixed-case\">EPIYA\u2010D<\/jats:styled-content> sequence, most of which were of <jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content> subtype. Three isolates from patients with chronic gastritis possessed the <jats:styled-content style=\"fixed-case\">EPIYA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> segment. The sequences flanking the <jats:styled-content style=\"fixed-case\">EPIYA<\/jats:styled-content> motifs contained polymorphisms at seven residues, among which amino acid positions 878 and 879 had a statistically significant association with gastric cancer (<jats:italic>P<\/jats:italic>\u2009=\u20090.021). Amino acid change from glycine to aspartic acid at residue 968 was present only in patients with gastric cancer (4\/20) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Most <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u2009pylori<\/jats:italic> strains present in our study are of the <jats:styled-content style=\"fixed-case\">CagA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ABD<\/jats:styled-content> subtype. Polymorphisms at amino acids 878 and 879 flanking the <jats:styled-content style=\"fixed-case\">EPIYA<\/jats:styled-content>\u2010A motif are statistically associated with gastric cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.12056","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T12:53:12Z","timestamp":1363870392000},"page":"358-365","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Amino acid polymorphisms flanking the <scp>EPIYA<\/scp>\u2010A motif of <i><scp>H<\/scp>elicobacter pylori<\/i>\u2005<scp>CagA C<\/scp>\u2010terminal region is associated with gastric cancer in <scp>E<\/scp>ast <scp>C<\/scp>hina: Experience from a single center"],"prefix":"10.1111","volume":"14","author":[{"given":"Chun Yan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai Jun","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin Xin","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hai","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for microRNA Biology and Biotechnology State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University  Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Jinling Hospital  Nanjing Jiangsu Province 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