{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:38:32Z","timestamp":1788871112147,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T00:00:00Z","timestamp":1430265600000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004386","name":"UNIVERSITY OF MALAYA","doi-asserted-by":"crossref","award":["RG460-12HTM"],"award-info":[{"award-number":["RG460-12HTM"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2015,4]]},"abstract":"\n Objective<\/jats:title>\n \n The study aimed to investigate the association between the interferon regulatory factor 5 (\n \n IRF5<\/jats:styled-content>\n <\/jats:italic>\n ) gene polymorphisms and the onset of\n C<\/jats:styled-content>\n rohn's disease (\n CD<\/jats:styled-content>\n ) in a\n M<\/jats:styled-content>\n alaysian cohort.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Genomic\n DNA<\/jats:styled-content>\n was extracted from blood samples collected from 91\n CD<\/jats:styled-content>\n patients and 100 healthy individuals via a conventional phenol\u2010chloroform extraction method. Screening of the four target single nucleotide polymorphisms (SNPs), including rs3807306, rs4728142, rs10954213 and rs11770589 was carried out in a real\u2010time polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) thermal cycler using\n TaqMan<\/jats:styled-content>\n genotyping assay. The genetic data obtained was subsequently subjected to statistical analysis to relate the\n SNP<\/jats:styled-content>\n s to the onset of\n CD<\/jats:styled-content>\n in the\n M<\/jats:styled-content>\n alaysian population. The genotyping assay and data were further validated selectively by conventional\n PCR<\/jats:styled-content>\n amplification of the\n SNP<\/jats:styled-content>\n sites and\n DNA<\/jats:styled-content>\n sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The rs3807306\n G<\/jats:styled-content>\n allele was a risk factor for\n CD<\/jats:styled-content>\n (OR 2.3630,\n P<\/jats:italic>\n = 0.00004), whereas the homozygous\n T<\/jats:styled-content>\n genotype was protective against the disease (OR 0.2038,\n P<\/jats:italic>\n = 0.00004). The heterozygous\n A\/G<\/jats:styled-content>\n genotype of rs10954213 was significantly associated with CD (OR 4.319,\n P<\/jats:italic>\n = 0.0377). On the other hand, the homozygous\n A<\/jats:styled-content>\n and heterozygous\n A\/G<\/jats:styled-content>\n genotypes of the rs11770589 were significant in the controls (OR 0.4242,\n P<\/jats:italic>\n = 0.0166) and patients (OR 2.000,\n P<\/jats:italic>\n = 0.0179), respectively. In the ethnic\u2010stratification analysis, the rs11770589 homozygous A genotype was protective in Indians (OR 0.1551,\n P<\/jats:italic>\n = 0.0112).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n \n IRF5<\/jats:styled-content>\n <\/jats:italic>\n gene polymorphisms may play a role in the development of\n CD<\/jats:styled-content>\n in the Malaysian population.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/1751-2980.12229","type":"journal-article","created":{"date-parts":[[2015,1,7]],"date-time":"2015-01-07T03:56:26Z","timestamp":1420602986000},"page":"205-216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Association between genetic polymorphisms in interferon regulatory factor 5 (\n IRF5<\/i>\n ) gene and\n M<\/scp>\n alaysian patients with\n C<\/scp>\n rohn's disease"],"prefix":"10.1111","volume":"16","author":[{"given":"Kek Heng","family":"Chua","sequence":"first","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lay Hoong","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei Ching","family":"Khor","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Way Seah","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Hilmi","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khean Lee","family":"Goh","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boon Pin","family":"Kee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur 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