{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:13:52Z","timestamp":1740147232805,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,8,17]],"date-time":"2015-08-17T00:00:00Z","timestamp":1439769600000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministerstwo Nauki i Szkolnictwa Wy\u017cszego","doi-asserted-by":"publisher","award":["N402 3073 36"],"award-info":[{"award-number":["N402 3073 36"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the effect of <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>elicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) eradication on the expression level of the <jats:italic><jats:styled-content style=\"fixed-case\">FHIT<\/jats:styled-content><\/jats:italic> gene and its methylation status in the gastric mucosa of dyspeptic patients with or without a family history of gastric cancer (<jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In all, 31 patients with <jats:italic>H. pylori<\/jats:italic> infection including 13 with <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content> were enrolled in the study. The effectiveness of <jats:italic>H. pylori<\/jats:italic> eradication were confirmed by <jats:styled-content style=\"fixed-case\">UBT<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">RUT<\/jats:styled-content> and multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (the presence of selected <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>. pylori<\/jats:italic> strains) for biopsy samples from the antrum and corpus. Histopathological assessment was also performed. The expression of <jats:styled-content style=\"fixed-case\"><jats:italic>FHIT<\/jats:italic> mRNA<\/jats:styled-content> was determined by quantitative reverse transcription\u2010polymerase chain reaction and the methylation status of the <jats:italic><jats:styled-content style=\"fixed-case\">FHIT<\/jats:styled-content><\/jats:italic> promoter was assessed by methylation\u2010specific polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>. pylori<\/jats:italic> eradication, the improvement of inflammation from superficial gastritis to normal mucosa (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2192<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>) was observed in 39% of the patients without <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content> and in 54% of those with <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\"><jats:italic>FHIT<\/jats:italic><\/jats:styled-content> mRNA expression was increased in patients without <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content> after <jats:italic>H. pylori<\/jats:italic> eradication (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), while there was no statistically significant change in gene methylation status after <jats:italic>H. pylori<\/jats:italic> eradication (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). For the samples from those with <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content>, the <jats:styled-content style=\"fixed-case\"><jats:italic>FHIT<\/jats:italic> mRNA<\/jats:styled-content> expression was not significantly changed and the methylation status fluctuated evenly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>H. pylori<\/jats:italic> eradication results in the improvement of gastric mucosal inflammation and histopathological non\u2010atrophic changes. The <jats:italic><jats:styled-content style=\"fixed-case\">FHIT<\/jats:styled-content><\/jats:italic> gene expression is increased in patients without <jats:styled-content style=\"fixed-case\">FHGC<\/jats:styled-content>, which may contribute to the prevention of <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.12252","type":"journal-article","created":{"date-parts":[[2015,5,5]],"date-time":"2015-05-05T09:07:07Z","timestamp":1430816827000},"page":"385-394","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The influence of <i><scp>H<\/scp>elicobacter pylori<\/i> eradication on the expression and methylation status of the <i><scp>FHIT<\/scp><\/i> gene in non\u2010cancerous gastric mucosa of dyspeptic patients"],"prefix":"10.1111","volume":"16","author":[{"given":"Lukasz","family":"Peczek","sequence":"first","affiliation":[{"name":"Department of Bioorganic Chemistry Centre of Molecular and Macromolecular Studies Polish Academy of Sciences  Lodz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karolina","family":"Zuk","sequence":"additional","affiliation":[{"name":"Department of Bioorganic Chemistry Centre of Molecular and Macromolecular Studies Polish Academy of Sciences  Lodz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krystyna","family":"Stec\u2010Michalska","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Medical University of Lodz  Lodz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Medrek","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Medical University of Lodz  Lodz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Nawrot","sequence":"additional","affiliation":[{"name":"Department of Bioorganic Chemistry Centre of Molecular and Macromolecular Studies Polish Academy of Sciences  Lodz Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"385","volume-title":"Helicobacter Pylori. 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