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This study aimed to explore the role of miR\u201030c and miR\u2010193 in liver fibrosis.<\/jats:p><\/jats:sec>Methods<\/jats:title>The regulation of miRNAs in carbon tetrachloride\u2010induced liver fibrosis was analyzed by microarray. Expression patterns of miR<\/jats:styled-content>\u2010193 and miR<\/jats:styled-content>\u201030c were further confirmed in fibrotic liver samples obtained from two murine models of hepatic fibrosis and human tissues. On a functional level, miRNA<\/jats:styled-content> levels were analyzed in the context of transforming growth factor (TGF<\/jats:styled-content>\u2010\u03b2) mediated activation of hepatic stellate cells (HSC<\/jats:styled-content>s). Finally, predicted targets were assessed for their roles in fibrosis by transfecting murine HSC<\/jats:styled-content>s with miRNA<\/jats:styled-content> mimics.<\/jats:p><\/jats:sec>Results<\/jats:title>Microarray analysis in murine fibrotic livers revealed a panel of 44 dysregulated miRNA<\/jats:styled-content>s. In addition to previously established miRNAs known to be regulated in liver fibrosis in a TGF\u2010\u03b2\u2010<\/jats:styled-content>dependent manner (e.g., miR<\/jats:styled-content>\u201029, miR<\/jats:styled-content>\u2010133), miR<\/jats:styled-content>\u2010193 and miR<\/jats:styled-content>\u201030c were observed to be specifically downregulated not only in experimental hepatofibrogenesis but also in human liver fibrosis, while they showed a reciprocal expression pattern after recovery from liver fibrosis. Functional experiments confirmed the TGF<\/jats:styled-content>\u2010\u03b2\u2010dependent downregulation of these respective new miRNA<\/jats:styled-content>s in HSC<\/jats:styled-content>s. Finally, we identified TGF<\/jats:italic><\/jats:styled-content>\u2010\u03b22<\/jats:italic> and SNAIL<\/jats:italic><\/jats:styled-content>1<\/jats:italic>, important regulators of extracellular matrix, as potential target genes of miR<\/jats:styled-content>\u2010193 and miR<\/jats:styled-content>\u201030 in liver fibrosis.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results suggest that miR<\/jats:styled-content>\u201030 and miR<\/jats:styled-content>\u2010193 are members of a network of miRNA<\/jats:styled-content>s modifying the TGF<\/jats:styled-content>\u2010\u03b2\u2010dependent regulation of extracellular matrix\u2010related genes in HSC<\/jats:styled-content>s in the manifestation and resolution of liver fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.12266","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T10:06:58Z","timestamp":1435572418000},"page":"513-524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["miR<\/scp>\u201030c and miR<\/scp>\u2010193 are a part of the TGF<\/scp>\u2010\u03b2\u2010dependent regulatory network controlling extracellular matrix genes in liver fibrosis"],"prefix":"10.1111","volume":"16","author":[{"given":"Sanchari","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Medicine III University of Aachen (RWTH) Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Benz","sequence":"additional","affiliation":[{"name":"Department of Medicine III University of Aachen (RWTH) Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Vargas 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