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Serum CCL5 expression was measured by an enzyme\u2010linked immunosorbent assay (ELISA). C57BL\/6 wild\u2010type (WT) and <jats:italic>Ccl<\/jats:italic>5\u2010knockout (<jats:italic>Ccl5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup>) mice were utilized to conduct the diethylnitrosamine\u2010induced HCC model. The immune cell population was determined by flow cytometry, and peripheral serum immunoglobulin M (IgM) level was quantified by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CCL5 expression was low in HCC tissue and peripheral blood compared with adjacent non\u2010cancerous tissues or controls, and its expression was correlated with the overall survival, cancer recurrence and distant metastasis. In the HCC mouse model, liver\u2010to\u2010body weight ratio was of the <jats:italic>Ccl5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> group were higher than that of the WT group. Moreover, compared with the WT mice, the number of B cells in the tumor tissue of the <jats:italic>Ccl5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice was lower, while there were no significant differences in the other immune cell populations. Furthermore, serum IgM level of the <jats:italic>Ccl5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice was significantly lower than that of the WT mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CCL5 expression is decreased in HCC tissues. CCL5 deficiency reduces B cell recruitment and decreases IgM secretion in HCC, potentially leading to tumor progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.12997","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T12:14:51Z","timestamp":1620821691000},"page":"433-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>C\u2010C<\/scp> chemokine hepatocellular carcinoma motif ligand 5<scp>\u2010deficiency<\/scp> promotes hepatocellular carcinoma progression by affecting <scp>B<\/scp> cell recruitment"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-1994","authenticated-orcid":false,"given":"Xiang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Hepatic Surgery, Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"},{"name":"Central Laboratory, Department of Liver Diseases 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