{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T07:06:03Z","timestamp":1763535963248,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81200272","81570490"],"award-info":[{"award-number":["81200272","81570490"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate whether the neuropsin pathway in the amygdala and stomach may participate in the development of anxiety\u2010related gastric hypersensitivity, and whether electroacupuncture (EA) at the Zusanli acupoint could improve this condition by regulating such pathway in the rat model of functional dyspepsia (FD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 48 SD rats were randomly divided into the control group, FD model group and FD\u2009+\u2009EA group (stimulation at Zusanli acupoint for 30\u2009min daily for 7 consecutive days). Abdominal withdrawal reflex (AWR) score and open field test were used to evaluate visceral hypersensitivity and anxiety\u2010like disorder, respectively. Electrical activity in the amygdala nucleus in each group was recorded by extracellular electrophysiology. Neuropsin and serpinb6 protein expressions in the amygdala and stomach were detected by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AWR score in the FD group increased but did not differ after EA therapy than that in the contro group. Both the center square entries and center entries ratio in the FD group were lower than those in the control and FD\u2009+\u2009EA groups. The total number and frequency of amygdala nucleus discharges induced by gastric distension in the FD group were significantly higher than those in the control and FD\u2009+\u2009EA groups. Expression of neuropsin increased and that of serpinb6 decreased in the gastric mucosa and amygdale in the FD group, while no change was observed in gastric mucosa after EA therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>EA stimulation at the Zusanli acupoint may improve visceral hypersensitivity and anxiety in FD rats through the neuropsin\/serpinb6 pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.13089","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T10:29:56Z","timestamp":1645180196000},"page":"149-156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The role of amygdala neuropsin\/serpinb6 pathway in the regulation of gastric hypersensitivity and anxiety by electroacupuncture"],"prefix":"10.1111","volume":"23","author":[{"given":"Fei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei Province China"},{"name":"Department of Gastroenterology First Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Union Hospital, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University  Wuhan Hubei Province 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