{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T21:18:09Z","timestamp":1783977489589,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170562"],"award-info":[{"award-number":["82170562"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7232199"],"award-info":[{"award-number":["7232199"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to explore the efficacy and safety of tailored therapy guided by genotypic resistance in the first\u2010line treatment of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) infection in treatment\u2010naive patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gastric mucosal specimens were taken during gastroscopy, and main mutations of clarithromycin\u2010 and levofloxacin\u2010resistant genes were detected by polymerase chain reaction (PCR). Sensitive antibiotics were selected individually for treating <jats:italic>H. pylori<\/jats:italic> infection with tailored bismuth\u2010containing quadruple therapy (BQT) consisting of esomeprazole 20\u2009mg twice daily, bismuth potassium citrate 220\u2009mg twice daily, amoxicillin 1\u2009g twice daily, and clarithromycin 500\u2009mg twice daily, or levofloxacin 500\u2009mg once daily, or metronidazole 400\u2009mg four times daily. Safety and patient compliance were assessed 1\u20133\u2009days after eradication. Treatment outcome was evaluated by urea breath test 4\u20138\u2009weeks after eradication.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred and thirty\u2010two treatment\u2010naive patients with <jats:italic>H. pylori<\/jats:italic> infection were included. PCR results suggested resistance rates of 47.7% and 34.9% for clarithromycin and levofloxacin, respectively, and a dual resistance rate of 18.2%. Eradication rates of tailored BQT were 87.1% and 95.8% by intention\u2010to\u2010treat (ITT) analysis and per\u2010protocol (PP) analysis, respectively. There was no statistically significant difference in the efficacy of 7\u2010day clarithromycin\u2010containing, 7\u2010day levofloxacin\u2010containing, and 14\u2010day full\u2010dose metronidazole\u2010containing BQT (ITT analysis: <jats:italic>P<\/jats:italic>\u2009=\u20090.488; PP analysis: <jats:italic>P<\/jats:italic> =\u20090.833). The incidence of adverse events was 19.7%, and patient compliance was 97.7%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Tailored BQT guided by genotypic resistance can achieve satisfactory efficacy, safety, and patient compliance in the first\u2010line treatment of <jats:italic>H. pylori<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.13250","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T11:10:55Z","timestamp":1707304255000},"page":"36-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tailored therapy guided by genotypic resistance of clarithromycin and levofloxacin detected by polymerase chain reaction in the first\u2010line treatment of <i>Helicobacter pylori<\/i> infection"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4687-8455","authenticated-orcid":false,"given":"Cai Ling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9763-0895","authenticated-orcid":false,"given":"Kai","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Beijing No. 6 Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao Jun","family":"Suo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue Li","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Lu","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Yan","family":"Shi","sequence":"additional","affiliation":[{"name":"Research Center of Clinical Epidemiology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Ya","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Qiang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Peking University Third Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-309252"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-327745"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000002546"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2016.563"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0000000000001719"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.07.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2014.08.038"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i9.2786"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-06"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.962063"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12467"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13099\u2010020\u201000373\u20106"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S305996"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.861626"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6150628"},{"issue":"1","key":"e_1_2_7_17_1","first-page":"37","article-title":"Application of PCR\u2013Sanger sequencing in individualized treatment of Helicobacter pylori infection","volume":"43","author":"Zhou QJ","year":"2021","journal-title":"Zhejiang Med"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(23)00097-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199610000-00001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12475"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312288"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12762"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.477"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12634"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05243-11"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.596371"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12071135"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(01)00468-X"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12299"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2021.03.006"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12958"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2021.10.011"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756284820952596"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00744-09"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.14383"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.5009\/gnl18079"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.04.006"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12935"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2022.2017280"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2022.12.019"}],"container-title":["Journal of Digestive Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-2980.13250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T02:11:10Z","timestamp":1708999870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1751-2980.13250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/1751-2980.13250"],"URL":"https:\/\/doi.org\/10.1111\/1751-2980.13250","archive":["Portico"],"relation":{},"ISSN":["1751-2972","1751-2980"],"issn-type":[{"value":"1751-2972","type":"print"},{"value":"1751-2980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}