{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:10:20Z","timestamp":1793243420058,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100605"],"award-info":[{"award-number":["82100605"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Digest Diseases"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Artesunate (ART) is a water\u2010soluble derivative of artemisinin, which has shown anti\u2010inflammatory, anti\u2010tumor, and immunomodulating effects. We aimed to investigate the potential therapeutic effects and mechanisms of ART in metabolic dysfunction\u2010associated steatohepatitis (MASH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The mice were randomly divided into the control group, high\u2010fat, high\u2010cholesterol diet\u2010induced MASH group, and the MASH treated with ART (30\u2009mg\/kg once daily) group. Liver enzymes, lipids, and histological features were compared among groups. The molecular mechanisms were studied by transcriptomic and lipidomics analyses of liver tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mice of the MASH group had significantly increased hepatic fat deposition and inflammation in terms of biochemical indicators and pathological manifestations than the control group. The ART\u2010treated group had improved plasma liver enzymes and hepatic cholesterol, especially at week 4 of intervention (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). A total of 513 differentially expressed genes\u00a0and 59 differentially expressed lipids were identified in the MASH group and the MASH+ART group. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment test showed that ART regulated glycerolipid metabolism pathway and enhanced fatty acid degradation. Peroxisome proliferator\u2010activated receptor (PPAR)\u2010\u03b1 acted as a key transcription factor in the treatment of MASH with ART, which was confirmed by cell experiment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ART significantly improved fat deposition and inflammatory manifestations in MASH mice, with potential therapeutic effects. The mechanism of artemisinin treatment for MASH may involve extensive regulation of downstream genes by upstream transcription factors, such as PPAR\u2010\u03b1, to restore hepatic lipid homeostasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1751-2980.13311","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T05:04:56Z","timestamp":1725944696000},"page":"490-503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Insight into the therapeutic effects of artesunate in relieving metabolic\u2010associated steatohepatitis from transcriptomic and lipidomics analyses"],"prefix":"10.1111","volume":"25","author":[{"given":"Jing","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Xinhua Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Key Lab of Pediatric Gastroenterology and Nutrition  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei Jie","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xinhua Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Key Lab of Pediatric Gastroenterology and Nutrition  Shanghai China"},{"name":"Department of Gastroenterology Ningbo No. 2 Hospital  Ningbo Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian Yi","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xinhua Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Key Lab of Pediatric Gastroenterology and Nutrition  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Xinhua Hospital, Shanghai Jiao Tong University School 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