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In our study, spinosad was used as an example to establish a\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    strategy by multi\u2010scale analysis, which included a reactor, cell and gene scales. We changed\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    conditions that are related to the characteristics of cell metabolism (glucose consumption rate, biomass accumulation and spinosad production). Consequently, cell growth was promoted by maintaining\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    at 40% in the first 24\u2009h and subsequently increasing\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    to 50% in 24\u2009h to 96\u2009h. In an in\u2010depth analysis of the key enzyme genes (\n                    <jats:italic>gtt<\/jats:italic>\n                    ,\n                    <jats:italic>spn<\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    ,\n                    <jats:italic>spn<\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>\n                    and\n                    <jats:italic>spn<\/jats:italic>\n                    \u2005\n                    <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>\n                    ), expression of spinosad and specific Adenosine Triphosphate (\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ), the spinosad yield was increased by regulating\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    to 30% within 96\u2009h to 192\u2009h and then changing it to 25% in 192\u2009h to 240\u2009h. Under the four\u2010phase\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    strategy, spinosad yield increased by 652.1%, 326.1%, 546.8%, and 781.4% compared with the yield obtained under constant\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    control at 50%, 40%, 30%, and 20% respectively. The proposed method provides a novel way to develop a precise\n                    <jats:styled-content style=\"fixed-case\">DO<\/jats:styled-content>\n                    strategy for fermentation.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.12264","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T14:58:25Z","timestamp":1427381905000},"page":"561-568","source":"Crossref","is-referenced-by-count":18,"title":["Four\u2010stage dissolved oxygen strategy based on multi\u2010scale analysis for improving spinosad yield by\n                    <scp>\n                      <i>S<\/i>\n                    <\/scp>\n                    <i>accharopolyspora spinosa<\/i>\n                    \u2005\n                    <scp>ATCC<\/scp>\n                    49460"],"prefix":"10.1111","volume":"8","author":[{"given":"Yun","family":"Bai","sequence":"first","affiliation":[{"name":"Institute of Resource Biology and Biotechnology Department of Biotechnology 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 Wuhan 430074 China"},{"name":"Key Laboratory of Molecular Biophysics Ministry of Education Huazhong University of Science and Technology  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Min","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Resource Biology and Biotechnology Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"},{"name":"Key Laboratory of Molecular Biophysics Ministry of Education Huazhong University of Science and Technology  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Tong","sequence":"additional","affiliation":[{"name":"Institute of Resource Biology and Biotechnology Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"},{"name":"Key Laboratory of Molecular Biophysics Ministry of Education Huazhong University of Science and Technology  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Resource Biology and Biotechnology Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"},{"name":"Key Laboratory of Molecular Biophysics Ministry of Education Huazhong University of Science and Technology  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long\u2010Jiang","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Resource Biology and Biotechnology Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology  Wuhan 430074 China"},{"name":"Key Laboratory of Molecular Biophysics Ministry of Education Huazhong University of Science and Technology  Wuhan 430074 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