{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:10:43Z","timestamp":1790007043029,"version":"4.0.1"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,7]],"date-time":"2015-12-07T00:00:00Z","timestamp":1449446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Suomen Akatemia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Suomen Kulttuurirahasto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004012","name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006706","name":"Tampereen Tuberkuloosis\u00e4\u00e4ti\u00d6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006706","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Summary<\/jats:title>\n \n Trichinellosis is a helminthic infection where different species of\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n nematodes are the causative agents. Several molecular assays have been designed to aid diagnostics of trichinellosis. These assays are mostly complex and expensive. The genomes of\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n species contain certain parasite\u2010specific genes, which can be detected by polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) methods. We selected \u03b2\u2010carbonic anhydrase (\n \u03b2\u2010<\/jats:italic>\n \n CA<\/jats:italic>\n <\/jats:styled-content>\n ) gene as a target, because it is present in many parasites genomes but absent in vertebrates. We developed a novel \u03b2\u2010\n CA<\/jats:styled-content>\n gene\u2010based method for detection of\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n larvae in biological samples. We first identified a \u03b2\u2010\n CA<\/jats:styled-content>\n protein sequence from\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella spiralis<\/jats:italic>\n by bioinformatic tools using \u03b2\u2010\n CAs<\/jats:styled-content>\n from\n \n C<\/jats:italic>\n <\/jats:styled-content>\n aenorhabditis elegans<\/jats:italic>\n and\n \n D<\/jats:italic>\n <\/jats:styled-content>\n rosophila melanogaster<\/jats:italic>\n . Thereafter, 16 sets of designed primers were tested to detect \u03b2\u2010\n CA<\/jats:styled-content>\n genomic sequences from three species of\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n , including\n \n T<\/jats:italic>\n <\/jats:styled-content>\n .\u2009spiralis<\/jats:italic>\n ,\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n \u2005\n \n pseudospiralis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n T<\/jats:italic>\n <\/jats:styled-content>\n richinella<\/jats:italic>\n \u2005\n \n nativa<\/jats:italic>\n <\/jats:styled-content>\n . Among all 16 sets of designed primers, the primer set\n N<\/jats:styled-content>\n o. 2 efficiently amplified \u03b2\u2010\n CA<\/jats:styled-content>\n genomic sequences from\n \n T<\/jats:italic>\n <\/jats:styled-content>\n .\u2009spiralis<\/jats:italic>\n ,\n \n T<\/jats:italic>\n <\/jats:styled-content>\n .\u2009pseudospiralis<\/jats:italic>\n and\n \n T<\/jats:italic>\n <\/jats:styled-content>\n .\u2009nativa<\/jats:italic>\n without any false\u2010positive amplicons from other parasite samples including\n \n T<\/jats:italic>\n <\/jats:styled-content>\n oxoplasma gondii<\/jats:italic>\n ,\n \n T<\/jats:italic>\n <\/jats:styled-content>\n oxocara cati<\/jats:italic>\n and\n \n P<\/jats:italic>\n <\/jats:styled-content>\n arascaris equorum<\/jats:italic>\n . This robust and straightforward method could be useful for meat inspection in slaughterhouses, quality control by food authorities and medical laboratories.\n <\/jats:p>","DOI":"10.1111\/1751-7915.12327","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T12:45:24Z","timestamp":1449578724000},"page":"172-179","source":"Crossref","is-referenced-by-count":15,"title":["Innovative molecular diagnosis of\n \n T<\/i>\n <\/scp>\n richinella<\/i>\n species based on \u03b2\u2010carbonic anhydrase genomic sequence"],"prefix":"10.1111","volume":"9","author":[{"given":"Reza","family":"Zolfaghari Emameh","sequence":"first","affiliation":[{"name":"School of Medicine University of Tampere FI\u201033520 Tampere Finland"},{"name":"BioMediTech University of Tampere FI\u201033520 Tampere Finland"},{"name":"Fimlab Laboratories Ltd and Tampere University Hospital Biokatu 4 FI\u201033520 Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Kuuslahti","sequence":"additional","affiliation":[{"name":"School of Medicine University of Tampere FI\u201033520 Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anu","family":"N\u00e4reaho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Faculty of Veterinary Medicine University of Helsinki FI\u201000014 Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"Sukura","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Faculty of Veterinary Medicine University of Helsinki FI\u201000014 Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Parkkila","sequence":"additional","affiliation":[{"name":"School of Medicine University of Tampere FI\u201033520 Tampere Finland"},{"name":"Fimlab Laboratories Ltd and Tampere University Hospital Biokatu 4 FI\u201033520 Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050556"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2202.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2011.02.008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja061574s"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.2807\/esm.03.08.00120-en","article-title":"History of trichinellosis outbreaks linked to horse meat consumption 1975\u20131998","volume":"3","author":"Ancelle T.","year":"1998","journal-title":"Euro Surveill"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X09359830"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0971-x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/89.8.631"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.08.015"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02521.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-009-9857-z"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39375-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2009.08.026"},{"key":"e_1_2_7_15_1","first-page":"221","article-title":"Chronic trichinosis. 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