{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:21:05Z","timestamp":1785475265142,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T00:00:00Z","timestamp":1463616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Cyanides are highly toxic and produced by various microorganisms as defence strategy or to increase their competitiveness. As degradation is the most efficient way of detoxification, some microbes developed the capability to use cyanides as carbon and nitrogen source. However, it is not clear if this potential also helps to lower cyanide concentrations in roadside soils where deicing salt application leads to significant inputs of ferrocyanide. The question remains if biodegradation in soils can occur without previous photolysis. By conducting a microcosm experiment using soils with\/without pre\u2010exposition to road salts spiked with\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010labelled ferrocyanide, we were able to confirm biodegradation and in parallel to identify bacteria using ferrocyanide as C source via\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    stable isotope probing\n                    <jats:styled-content style=\"fixed-case\">(DNA\u2010SIP<\/jats:styled-content>\n                    ),\n                    <jats:styled-content style=\"fixed-case\">TRFLP<\/jats:styled-content>\n                    fingerprinting and pyrosequencing. Bacteria assimilating\n                    <jats:sup>13<\/jats:sup>\n                    C were highly similar in the pre\u2010exposed soils, belonging mostly to\n                    <jats:italic>Actinomycetales<\/jats:italic>\n                    (\n                    <jats:italic>Kineosporia<\/jats:italic>\n                    ,\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Micromonosporaceae<\/jats:italic>\n                    ). In the soil without pre\u2010exposition, bacteria belonging to Aci\n                    <jats:italic>dobacteria<\/jats:italic>\n                    (Gp3, Gp4, Gp6),\n                    <jats:italic>Gemmatimonadetes<\/jats:italic>\n                    (\n                    <jats:italic>Gemmatimonas<\/jats:italic>\n                    ) and\n                    <jats:italic>Gammaproteobacteria<\/jats:italic>\n                    (\n                    <jats:italic>Thermomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Xanthomonadaceae<\/jats:italic>\n                    ) used ferrocyanide as C source but not the present\n                    <jats:italic>Actinomycetales<\/jats:italic>\n                    . This indicated that (i) various bacteria are able to assimilate ferrocyanide\u2010derived C and (ii) long\u2010term exposition to ferrocyanide applied with deicing salts leads to\n                    <jats:italic>Actinomycetales<\/jats:italic>\n                    outcompeting other microorganisms for the use of ferrocyanide as C source.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.12362","type":"journal-article","created":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T17:51:55Z","timestamp":1463680315000},"page":"502-513","source":"Crossref","is-referenced-by-count":12,"title":["Long\u2010term ferrocyanide application via deicing salts promotes the establishment of Actinomycetales assimilating ferrocyanide\u2010derived carbon in soil"],"prefix":"10.1111","volume":"9","author":[{"given":"Silvia","family":"Gschwendtner","sequence":"first","affiliation":[{"name":"Research Unit Environmental Genomics Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Ingolst\u00e4dter Landstra\u00dfe 1 Neuherberg 85764 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Mansfeldt","sequence":"additional","affiliation":[{"name":"Department Geowissenschaften, Bodengeographie\/Bodenkunde Universit\u00e4t zu K\u00f6ln Albertus\u2010Magnus\u2010Platz K\u00f6ln 50923 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Kublik","sequence":"additional","affiliation":[{"name":"Research Unit Environmental Genomics Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Ingolst\u00e4dter Landstra\u00dfe 1 Neuherberg 85764 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelia","family":"Touliari","sequence":"additional","affiliation":[{"name":"Research Unit Environmental Genomics Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Ingolst\u00e4dter Landstra\u00dfe 1 Neuherberg 85764 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Buegger","sequence":"additional","affiliation":[{"name":"Institute of Biochemical Plant Pathology Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Ingolst\u00e4dter Landstra\u00dfe 1 Neuherberg 85764 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schloter","sequence":"additional","affiliation":[{"name":"Research Unit Environmental Genomics Helmholtz Zentrum M\u00fcnchen German Research Center for Environmental Health (GmbH) Ingolst\u00e4dter Landstra\u00dfe 1 Neuherberg 85764 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,19]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"213","volume-title":"Proceedings of the Residual Management by Land Disposal, Hazardous Waste Research Symposium","author":"Alesii 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