{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:28:14Z","timestamp":1762280894504,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G.0163.11"],"award-info":[{"award-number":["G.0163.11"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    American Foulbrood, caused by\n                    <jats:italic>Paenibacillus larvae<\/jats:italic>\n                    , is the most severe bacterial disease of honey bees (\n                    <jats:italic>Apis mellifera<\/jats:italic>\n                    ). To perform genotyping of\n                    <jats:italic>P.\u00a0larvae<\/jats:italic>\n                    in an epidemiological context, there is a need of a fast and cheap method with a high resolution. Here, we propose Multiple Locus Variable number of tandem repeat Analysis (\n                    <jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>\n                    ).\n                    <jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>\n                    has been used for typing a collection of 209\n                    <jats:italic>P.\u00a0larvae<\/jats:italic>\n                    strains from which 23 different\n                    <jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>\n                    types could be identified. Moreover, the developed methodology not only permits the identification of the four Enterobacterial Repetitive Intergenic Consensus (\n                    <jats:styled-content style=\"fixed-case\">ERIC<\/jats:styled-content>\n                    ) genotypes, but allows also a discriminatory subdivision of the most dominant\n                    <jats:styled-content style=\"fixed-case\">ERIC<\/jats:styled-content>\n                    type I and\n                    <jats:styled-content style=\"fixed-case\">ERIC<\/jats:styled-content>\n                    type II genotypes. A biogeographical study has been conducted showing a significant correlation between\n                    <jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>\n                    genotype and the geographical region where it was isolated.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.12375","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T01:49:19Z","timestamp":1467337759000},"page":"772-781","source":"Crossref","is-referenced-by-count":14,"title":["Multiple Locus Variable number of tandem repeat Analysis: A molecular genotyping tool for\n                    <i>Paenibacillus larvae<\/i>"],"prefix":"10.1111","volume":"9","author":[{"given":"Tine","family":"Descamps","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Entomology and Bee Pathology Faculty of Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"De Smet","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Entomology and Bee Pathology Faculty of Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter","family":"Stragier","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Faculty of Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"De Vos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Faculty of Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk C.","family":"de Graaf","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Entomology and Bee Pathology Faculty of Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.1998.4748"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.7.3655-3660.2002"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.04.012"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1991.tb00608.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00873085"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2014.993095"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)80053-8"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00173.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12289"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02245-08"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-450"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.1994.11100856"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090914"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2001136"},{"key":"e_1_2_5_16_1","first-page":"D1","article-title":"TRDB\u2010The tandem repeats database","volume":"00","author":"Gelfand J.","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2009.06.015"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2003025"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.7551-7555.2005"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63928-0"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401561101"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2001146"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2005.10.010"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-1-270"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1128\/jb.59.2.153-155.1950","article-title":"Bacillus pulvifaciens (N. SP.), an organism associated with powdery scale of honeybee larvae","volume":"59","author":"Katznelson H.","year":"1950","journal-title":"J Bacteriol"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3179-3185.2001"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500096"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9342-y"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2008.06.010"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2008.09.003"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3095-3100.2005"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1901"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12625"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27125-0"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2006.00694.x"},{"key":"e_1_2_5_36_1","unstructured":"R Core Team(2015).R: A language and environment for statistical computing. URLhttps:\/\/www.R-project.org\/."},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.006"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01036-12"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01151-15"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.23.6990-6994.2003"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00986-07"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00001-06"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/00218839.2005.11101155"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1751-7915.12375","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.12375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:23:43Z","timestamp":1762280623000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.12375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/1751-7915.12375"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.12375","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"type":"print","value":"1751-7915"},{"type":"electronic","value":"1751-7915"}],"subject":[],"published":{"date-parts":[[2016,7]]}}}