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Databases and search strategies will struggle to cope and manual curation will not be sustainable during the scale\u2010up to the million\u2010microbial\u2010genome era. Microbial taxonomy will have to adapt to a situation in which most microorganisms are discovered and characterised through the analysis of sequences. Genome sequencing will become a routine approach in clinical and research laboratories, with fresh demands for interpretable user\u2010friendly outputs. The \u201cinternet of things\u201d will penetrate healthcare systems, so that even a piece of hospital plumbing might have its own IP address that can be integrated with pathogen genome sequences. Microbiome mania will continue, but the tide will turn from molecular barcoding towards metagenomics. Crowd\u2010sourced analyses will collide with cloud computing, but eternal vigilance will be the price of preventing the misinterpretation and overselling of microbial sequence data. Output from hand\u2010held sequencers will be analysed on mobile devices. Open\u2010source training materials will address the need for the development of a skilled labour force. As we boldly go into the third decade of the twenty\u2010first century, microbial sequence space will remain the final frontier!<\/jats:p>","DOI":"10.1111\/1751-7915.12389","type":"journal-article","created":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T10:25:54Z","timestamp":1471861554000},"page":"681-686","source":"Crossref","is-referenced-by-count":20,"title":["Microbial bioinformatics 2020"],"prefix":"10.1111","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1807-3657","authenticated-orcid":false,"given":"Mark J.","family":"Pallen","sequence":"first","affiliation":[{"name":"Microbiology and Infection Unit Warwick Medical School University of Warwick Coventry CV4 7AL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,29]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.07.008"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140829"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.01.001"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3103"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1752"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.02.004"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211115"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6850"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10063"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054673"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14486"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3239"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.054171-0"},{"key":"e_1_2_4_15_1","unstructured":"T.R. 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