{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:28:03Z","timestamp":1762280883434,"version":"build-2065373602"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T00:00:00Z","timestamp":1474243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31301116"],"award-info":[{"award-number":["31301116"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"Summary<\/jats:title>\n \n We aimed to assess the feasibility of enhancing the intestinal development of weaned rats using glucagon\u2010like peptide\u20102 (\n GLP<\/jats:styled-content>\n \u20102)\u2010expressing\n Saccharomyces cerevisiae<\/jats:italic>\n (\n S.\u00a0cerevisiae<\/jats:italic>\n ).\n GLP<\/jats:styled-content>\n \u20102\u2010expressing\n S.\u00a0cerevisiae<\/jats:italic>\n (\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n ) was generated using a recombinant approach. The diet of weaned rats was supplemented with the\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n strain. The average daily gain (\n ADG<\/jats:styled-content>\n ), the intestinal morphology and the activities of the digestive enzymes in the jejunum were tested to assess the influence of the\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n strain on intestinal development. The proliferation of rat enterocytes was also assessed in\u00a0vitro. The study revealed that the\n ADG<\/jats:styled-content>\n of the weaned rats that received\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n was significantly greater than that of the controls fed a basal diet (Control) and\n S.\u00a0cerevisiae<\/jats:italic>\n harbouring an empty vector (\n EV<\/jats:styled-content>\n \u2010\n SC<\/jats:styled-content>\n ) (\n P<\/jats:italic>\n \u00a0<\u00a00.05) but was equivalent to that of positive control rats fed recombinant human\n GLP<\/jats:styled-content>\n \u20102 (rh\u2010\n GLP<\/jats:styled-content>\n 2) (\n P<\/jats:italic>\n \u00a0>\u00a00.05). Furthermore,\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n significantly increased villous height (\n P<\/jats:italic>\n \u00a0<\u00a00.01) and digestive enzyme activity (\n P<\/jats:italic>\n \u00a0<\u00a00.05) in the jejunum. Immunohistochemistry analysis further affirmed that enterocyte proliferation was stimulated in rats fed the\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n strain, as indicated by the greater number of enterocytes stained with proliferative cell nuclear antigen (\n P<\/jats:italic>\n \u00a0<\u00a00.05). In vitro, the proliferation of rat enterocytes was also stimulated by\n GLP<\/jats:styled-content>\n \u20102 expressed by the\n GLP<\/jats:styled-content>\n 2\u2010\n SC<\/jats:styled-content>\n strain (\n P<\/jats:italic>\n \u00a0<\u00a00.01). Herein, the combination of the\n GLP<\/jats:styled-content>\n \u20102 approach and probiotic delivery constitute a possible dietary supplement for animals after weaning.\n <\/jats:p>","DOI":"10.1111\/1751-7915.12412","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T01:42:53Z","timestamp":1474249373000},"page":"846-857","source":"Crossref","is-referenced-by-count":5,"title":["Generation of glucagon\u2010like peptide\u20102\u2010expressing\n Saccharomyces cerevisiae<\/i>\n and its improvement of the intestinal health of weaned rats"],"prefix":"10.1111","volume":"9","author":[{"given":"Zhongwei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Intensive Care Unit West China Hospital Sichuan University Chengdu Sichuan 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Wu","sequence":"additional","affiliation":[{"name":"Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region Chengdu Sichuan 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Cao","sequence":"additional","affiliation":[{"name":"Medical School Chengdu University Chengdu Sichuan 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengdong","family":"Zhong","sequence":"additional","affiliation":[{"name":"Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Chengdu Sichuan 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit West China Hospital Sichuan University Chengdu Sichuan 610041 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