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While a handful of similar recombinases have been documented, recombineering efficiencies usually fall short of expectations for practical use. In this work, we aimed to find an efficient Rec\u03b2 homologue demonstrating activity in model soil bacterium\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    EM42. To this end, a genus\u2010wide protein survey was conducted to identify putative recombinase candidates for study. Selected novel proteins were assayed in a standardized test to reveal their ability to introduce the K43T substitution into the\n                    <jats:italic>rpsL<\/jats:italic>\n                    gene of\n                    <jats:italic>P.\u00a0putida<\/jats:italic>\n                    . An\n                    <jats:styled-content style=\"fixed-case\">ERF<\/jats:styled-content>\n                    superfamily protein, here termed Rec2, exhibited activity eightfold greater than that of the previous leading recombinase. To bolster these results, we demonstrated Rec2 ability to enter a range of mutations into the\n                    <jats:italic>pyrF<\/jats:italic>\n                    gene of\n                    <jats:italic>P.\u00a0putida<\/jats:italic>\n                    at similar frequencies. Our results not only confirm the utility of Rec2 as a Rec\u03b2 functional analogue within the\n                    <jats:italic>P.\u00a0putida<\/jats:italic>\n                    model system, but also set a complete workflow for deploying recombineering in other bacterial strains\/species. Implications range from genome editing of\n                    <jats:italic>P.\u00a0putida<\/jats:italic>\n                    for metabolic engineering to extended applications within other\n                    <jats:italic>Pseudomonads<\/jats:italic>\n                    \u2013 and beyond.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.12846","type":"journal-article","created":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T04:14:37Z","timestamp":1509596077000},"page":"176-188","source":"Crossref","is-referenced-by-count":45,"title":["A standardized workflow for surveying recombinases expands bacterial genome\u2010editing capabilities"],"prefix":"10.1111","volume":"11","author":[{"given":"Deirdre E.","family":"Ricaurte","sequence":"first","affiliation":[{"name":"Systems Biology Program Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus de Cantoblanco Madrid 28049 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