{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T11:54:07Z","timestamp":1769946847226,"version":"3.49.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T00:00:00Z","timestamp":1507248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013316","name":"SERDP","doi-asserted-by":"crossref","award":["ER-2301"],"award-info":[{"award-number":["ER-2301"]}],"id":[{"id":"10.13039\/100013316","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Two bacterial consortia were enriched from uncontaminated soil by virtue of their ability to grow on 1,4\u2010dioxane (dioxane) as a sole carbon and energy source. Their specific dioxane degradation rates at 30\u00b0C,\n                    <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\n                    \u00a0=\u00a07 (i.e. 5.7 to 7.1\u00a0g\u2010dioxane per g\u2010protein per day) were comparable to those of two dioxane\u2010metabolizing archetypes:\n                    <jats:italic>Pseudonocardia dioxanivorans<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\n                    1190 and\n                    <jats:italic>Mycobacterium dioxanotrophicus<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">PH<\/jats:styled-content>\n                    \u201006. Based on 16S\n                    <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>\n                    sequencing,\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    was the dominant genus. Acetylene inhibition tests suggest that dioxane degradation was mediated by monooxygenases. However,\n                    <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>\n                    analyses targeting the tetrahydrofuran\/dioxane monooxygenase gene (\n                    <jats:italic>thmA\/dxmA<\/jats:italic>\n                    ) (which is, to date, the only sequenced dioxane monooxygenase gene) were negative, indicating that other (as yet unknown) catabolic gene(s) were responsible.\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    sequence analyses also showed threefold to sevenfold enrichment of group 5 and group 6 soluble di\u2010iron monooxygenase (\n                    <jats:styled-content style=\"fixed-case\">SDIMO<\/jats:styled-content>\n                    ) genes relative to the original soil samples. Whereas biodegradation of trace levels of dioxane is a common challenge at contaminated sites, both consortia degraded dioxane at low initial concentrations (300\u00a0\u03bcg\u00a0l\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) below detectable levels (5\u00a0\u03bcg\u00a0l\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) in bioaugmented microcosms prepared with impacted groundwater. Overall, this work shows that dioxane\u2010degrading bacteria (and the associated natural attenuation potential) exist even in some uncontaminated soils, and may be enriched to broaden bioaugmentation options for sites experiencing insufficient dioxane catabolic capacity.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.12850","type":"journal-article","created":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T07:16:38Z","timestamp":1507274198000},"page":"189-198","source":"Crossref","is-referenced-by-count":52,"title":["1,4\u2010Dioxane\u2010degrading consortia can be enriched from uncontaminated soils: prevalence of\n                    <i>Mycobacterium<\/i>\n                    and soluble di\u2010iron monooxygenase genes"],"prefix":"10.1111","volume":"11","author":[{"given":"Ya","family":"He","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering Rice University Houston TX 77005 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