{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:20:47Z","timestamp":1772133647820,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31402078"],"award-info":[{"award-number":["31402078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccines, the third\u2010generation vaccines, were extensively studied. The attenuated\n                    <jats:italic>Salmonella choleraesuis<\/jats:italic>\n                    (\n                    <jats:italic>S.\u00a0choleraesuis<\/jats:italic>\n                    ) was widely focused as a carrier to deliver\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccines in the chromosome\u2013plasmid balanced\u2010lethal system. The efficacy of inhibin\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccine delivered by attenuated\n                    <jats:italic>S.\u00a0choleraesuis<\/jats:italic>\n                    was proved in mice and cows in our previous studies. In this study, the efficacy of inhibin\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccine was confirmed in rhesus monkeys. To further study the biodistribution and safety, the mice were immunized under laboratory conditions. The results of the rhesus monkeys showed the plasma IgA and IgG titres against inhibin were elevated, and the oestradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) and progesterone (P\n                    <jats:sub>4<\/jats:sub>\n                    ) levels were increased with immunizing inhibin\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccine. The biodistribution and safety assessment displayed the body weight, pathological change and haematology indexes where there is no significant difference between vaccinated mice and control. And the genomics analysis showed there was no integration of the inhibin gene into the mouse genome 2\u00a0months after immunization. This study indicated the inhibin\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    vaccine delivered by attenuated\n                    <jats:italic>S.\u00a0choleraesuis<\/jats:italic>\n                    was safe. And this vaccine was a potential means to improve their reproductive traits in primates and other animals.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13029","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T05:40:46Z","timestamp":1512452446000},"page":"248-256","source":"Crossref","is-referenced-by-count":5,"title":["The efficacy, biodistribution and safety of an inhibin\n                    <scp>DNA<\/scp>\n                    vaccine delivered by attenuated\n                    <i>Salmonella choleraesuis<\/i>"],"prefix":"10.1111","volume":"11","author":[{"given":"Wei\u2010zhen","family":"Chen","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying\u2010mei","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Helaiente biological science and Technology Co., Ltd Tianjin 301709 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