{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T18:27:14Z","timestamp":1781720834232,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004576","name":"Ministry of Agriculture and Rural Development","doi-asserted-by":"publisher","award":["12\u201005\u20100016"],"award-info":[{"award-number":["12\u201005\u20100016"]}],"id":[{"id":"10.13039\/501100004576","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Many types of crops are severely affected by at least one important bacterial disease. Chemical control of bacterial plant diseases in the field vastly relies on copper\u2010based bactericides, yet with limited efficacy. In this study, we explored the potential of two random peptide mixture (\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    ) models as novel crop protection agents. These unique peptide mixtures consist of random combination of\n                    <jats:sc>l<\/jats:sc>\n                    \u2010phenylalanine and\n                    <jats:sc>l<\/jats:sc>\n                    \u2010 or\n                    <jats:sc>d<\/jats:sc>\n                    \u2010lysine (\n                    <jats:styled-content style=\"fixed-case\">FK<\/jats:styled-content>\n                    \u201020 and FdK\u201020, respectively) along the 20 mer chain length of the peptides. Both\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    s displayed powerful bacteriostatic and bactericidal activities towards strains belonging to several plant pathogenic bacterial genera, for example,\n                    <jats:italic>Xanthomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Clavibacter<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas. In planta<\/jats:italic>\n                    studies in the glasshouse revealed that\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    s significantly reduced disease severity of tomato and kohlrabi plants infected with\n                    <jats:italic>Xanthomonas perforans<\/jats:italic>\n                    and\n                    <jats:italic>Xanthomonas campestris<\/jats:italic>\n                    pv.\n                    <jats:italic>campestris<\/jats:italic>\n                    respectively. Moreover,\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    effects on reduction in disease severity were similar to those exerted by the commercial copper\u2010based bactericide Kocide 2000 that was applied at a 12\u2010fold higher concentration of the active compound relative to the RPM treatments. Importantly, the two tested\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    compounds had no toxic effect on survival of bees and Caco\u20102 mammalian cells. This study demonstrates the potential of these innovative\n                    <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\n                    s to serve as crop protection agents against crop diseases caused by phytopathogenic bacteria.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13258","type":"journal-article","created":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T01:12:40Z","timestamp":1519780360000},"page":"1027-1036","source":"Crossref","is-referenced-by-count":42,"title":["Random peptide mixtures as new crop protection agents"],"prefix":"10.1111","volume":"11","author":[{"given":"Shiri","family":"Topman","sequence":"first","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"},{"name":"Department of Plant Pathology and Microbiology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafna","family":"Tamir\u2010Ariel","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heli","family":"Bochnic\u2010Tamir","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tal","family":"Stern Bauer","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharoni","family":"Shafir","sequence":"additional","affiliation":[{"name":"Department of Entomology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul","family":"Burdman","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3582-4029","authenticated-orcid":false,"given":"Zvi","family":"Hayouka","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Food Science and Nutrition The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot 76100 Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2002.15.7.701"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.09.010"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03043-10"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1094\/PD-89-1339"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1128\/aem.51.2.323-327.1986","article-title":"Generation and characterization of Tn5 insertion mutations in Pseudomonas syringae pv. tomato","volume":"51","author":"Cuppels D.A.","year":"1986","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11951"},{"key":"e_1_2_8_8_1","volume-title":"Encycl. Life Support Syst","author":"De La Fuente L.","year":"2011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161209788682325"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12184"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2573-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.45.062806.094321"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02759-10"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00092-5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja406231b"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201702313"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215039"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-81-714"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1078\/0723202042369884"},{"key":"e_1_2_8_20_1","first-page":"301","article-title":"Two simple media for the demonstration of pyocyanin and fluorescin","volume":"44","author":"King E.O.","year":"1954","journal-title":"J Lab Clin Med"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-012-0138-7"},{"key":"e_1_2_8_22_1","first-page":"e50854","article-title":"Establishing the minimal bactericidal concentration of an antimicrobial agent for planktonic cells (MBC\u2010P) and biofilm cells (MBC\u2010B)","volume":"83","author":"Mah T.F.","year":"2014","journal-title":"J Vis Exp"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-011-9878-z"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.121307.094843"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.1.184-190.1991"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.12.028"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi962507l"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03080.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-014-9649-9"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12244"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8093247"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2007022"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/417459a"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CC01438K"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2006.01456.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2012.00833.x"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.2000.90.2.191"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-02-12-0190-RE"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1751-7915.13258","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T17:40:15Z","timestamp":1762278015000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.13258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,28]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/1751-7915.13258"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.13258","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,28]]}}}