{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:02:06Z","timestamp":1791954126420,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Basic Research and Development Program","award":["2015CB150600"],"award-info":[{"award-number":["2015CB150600"]}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Sexual mating of compatible sporida is essential for\n                    <jats:italic>Sporisorium scitamineum<\/jats:italic>\n                    to form dikaryotic mycelia and then cause infection on sugarcane. Our previous work identified a\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp.\n                    <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>\n                    4 from a soil sample, which showed a promising biocontrol potential by inhibiting the mating of\n                    <jats:italic>S.\u00a0scitamineum<\/jats:italic>\n                    sporida and hyphal growth. In this study, we set to isolate the active compounds from\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp.\n                    <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>\n                    4 through solid fermentation. High\u2010performance liquid chromatography (\n                    <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\n                    ) separation coupling with bioassay showed that\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp.\n                    <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>\n                    4 produced a range of antimicrobial compounds. Two of the major components were purified following acetate extraction, silica gel and\n                    <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\n                    separation. Nuclear magnetic resonance (\n                    <jats:styled-content style=\"fixed-case\">NMR<\/jats:styled-content>\n                    ) and liquid chromatography\u2013mass spectrometry (\n                    <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    ) analysis identified these active compounds are 4\u2010hydroxybenzaldehyde and indole\u20103\u2010carbaldehyde respectively. Further analysis showed that the former compound only inhibited the hyphal growth of the fungus at a concentration of 3\u00a0\n                    <jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>\n                    , while the latter interfered the fungal sexual mating at a concentration of 0.6\u00a0\n                    <jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>\n                    and affected hyphal growth at a concentration of 2\u00a0\n                    <jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>\n                    . Treatment of corn plants with 3\u00a0\n                    <jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>\n                    indole\u20103\u2010carbaldehyde significantly inhibited corn smut infection, with a control rate up to 94%. Further analysis of the structure and activity relationship revealed that indole has a much stronger inhibitory activity against the fungal sexual mating than indole\u20103\u2010carbaldehyde. The results from this study provide new agents for control and prevention of the sugarcane smut disease, and the active compounds could also be used to probe the molecular mechanisms of fungal sexual mating.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13289","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T00:14:34Z","timestamp":1529626474000},"page":"107-117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<i>Pseudomonas<\/i>\n                    sp.\n                    <scp>ST<\/scp>\n                    4 produces variety of active compounds to interfere fungal sexual mating and hyphal growth"],"prefix":"10.1111","volume":"13","author":[{"given":"Shiyin","family":"Liu","sequence":"first","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"He","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuoqiao","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibin","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisheng","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfa","family":"Lv","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9774-330X","authenticated-orcid":false,"given":"Jianuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian\u2010Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Guangzhou China"},{"name":"Integrative Microbiology Research Centre South China Agricultural University Guangzhou 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