{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T19:05:35Z","timestamp":1783191935348,"version":"3.54.6"},"reference-count":78,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["1016249"],"award-info":[{"award-number":["1016249"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007917","name":"Agricultural Research Service","doi-asserted-by":"publisher","award":["59\u20108072\u20106\u2010001"],"award-info":[{"award-number":["59\u20108072\u20106\u2010001"]}],"id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    has been implicated in a number of outbreaks including the recent largest outbreak in South Africa. Current methods for prevention of foodborne\n                    <jats:italic>L.\u00a0monocytogenes<\/jats:italic>\n                    infection are inadequate, thus raising a need for an alternative strategy. Probiotic bioengineering is considered a prevailing approach to enhance the efficacy of probiotics for targeted control of pathogens. Here, the ability of\n                    <jats:italic>Lactobacillus casei<\/jats:italic>\n                    expressing the\n                    <jats:italic>L.\u00a0monocytogenes<\/jats:italic>\n                    invasion proteins Internalins A and B (\n                    <jats:italic>\n                      inl\n                      <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    <\/jats:italic>\n                    ) to prevent infection was investigated. The\n                    <jats:italic>\n                      inl\n                      <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    <\/jats:italic>\n                    operon was cloned and surface\u2010expressed on\n                    <jats:italic>L.\u00a0casei<\/jats:italic>\n                    resulting in a recombinant strain, Lbc\n                    <jats:sup>Inl<\/jats:sup>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>AB<\/jats:sup>\n                    <\/jats:styled-content>\n                    , and subsequently, its ability to inhibit adhesion, invasion and translocation of\n                    <jats:italic>L.\u00a0monocytogenes<\/jats:italic>\n                    through enterocyte\u2010like Caco\u20102 cells was examined. Cell surface expression of Inl\n                    <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    on the Lbc\n                    <jats:sup>Inl<\/jats:sup>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>AB<\/jats:sup>\n                    <\/jats:styled-content>\n                    was confirmed by Western blotting and immunofluorescence staining. The Lbc\n                    <jats:sup>Inl<\/jats:sup>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>AB<\/jats:sup>\n                    <\/jats:styled-content>\n                    strain showed significantly higher (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0.0001) adherence, invasion and translocation of Caco\u20102 cells than the wild\u2010type\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>casei<\/jats:italic>\n                    strain (Lbc\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>WT<\/jats:sup>\n                    <\/jats:styled-content>\n                    ), as well as reduced\n                    <jats:italic>L.\u00a0monocytogenes<\/jats:italic>\n                    adhesion, invasion and transcellular passage through the cell monolayer than Lbc\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>WT<\/jats:sup>\n                    <\/jats:styled-content>\n                    . Furthermore, pre\u2010exposure of Caco\u20102 cells to Lbc\n                    <jats:sup>Inl<\/jats:sup>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:sup>AB<\/jats:sup>\n                    <\/jats:styled-content>\n                    significantly reduced\n                    <jats:italic>L.\u00a0monocytogenes<\/jats:italic>\n                    \u2010induced cell cytotoxicity and epithelial barrier dysfunction. These results suggest that Inl\n                    <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\n                    \u2010expressing\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>casei<\/jats:italic>\n                    could be a potential practical approach for prevention of listeriosis.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13407","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T22:30:50Z","timestamp":1555367450000},"page":"715-729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>Lactobacillus casei<\/i>\n                    expressing Internalins A and B reduces\n                    <i>Listeria monocytogenes<\/i>\n                    interaction with Caco\u20102 cells\n                    <i>in\u00a0vitro<\/i>"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2554-8641","authenticated-orcid":false,"given":"Moloko G.","family":"Mathipa","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Genetics, and Microbiology University of Pretoria Pretoria South Africa"},{"name":"Molecular Food Microbiology Laboratory Department of Food Science Purdue University West Lafayette IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6212-2770","authenticated-orcid":false,"given":"Mapitsi S.","family":"Thantsha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Genetics, and Microbiology University of Pretoria Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3640-1554","authenticated-orcid":false,"given":"Arun K.","family":"Bhunia","sequence":"additional","affiliation":[{"name":"Molecular Food Microbiology Laboratory Department of Food Science Purdue University West Lafayette IN USA"},{"name":"Department of Comparative Pathobiology Purdue University West Lafayette IN USA"},{"name":"Purdue Institute of Inflammation, Immunology, and Infectious Disease Purdue University West Lafayette IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029910000877"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00538-18"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394598-3.00005-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4161\/bioe.23574"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01324-17"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00003-X"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010074"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00039-06"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4621825.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114516004037"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/505433"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00516-10"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.05.006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.2.5.17703"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.04.012"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00645"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00292.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2006-456"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700440104"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1079\/AHR2005111"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4238\/2015.December.23.36"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1517\/14740338.2014.872627"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.09.012"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.12.007"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.03.004"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.90992"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.4.1299-1308.1996"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.13.2.219-226.2006"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00160-18"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2005.10.003"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.02.012"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00825-09"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01553"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.036509-0"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020694"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/357964"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00411"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.01.055"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05153-11"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.05.011"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029277"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03574.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002635"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05009-0"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-015-1158-9"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10321"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2008.00024.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00010-9"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.7.975"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-275"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81070-3"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110560"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70870-9"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.57"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910307107"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-2-117"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.4161\/bbug.1.3.10665"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.10.4.696-701.2003"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.4.1263-1267.1992"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)36602-8"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.126"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26695-0"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000732"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-56836-2_21"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.P11101"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01136-4"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.2.169"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00141-0"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00993.x"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6918(03)00072-6"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.02085.x"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401024"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.03.013"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.931-936.2004"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1840"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01938-16"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b17-00657"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1751-7915.13407","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13407","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.13407","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111%2F1751-7915.13407","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13407","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:34:40Z","timestamp":1762256080000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.13407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,15]]},"references-count":78,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1111\/1751-7915.13407"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.13407","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,15]]},"assertion":[{"value":"2018-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}