{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:51:36Z","timestamp":1791823896979,"version":"4.3.4"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002723","name":"Allianz Industrie Forschung","doi-asserted-by":"publisher","award":["ZF4315807RH7"],"award-info":[{"award-number":["ZF4315807RH7"]}],"id":[{"id":"10.13039\/501100002723","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The detection and quantification of\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    (responsible for legionnaire's disease) in water samples can be achieved by various methods. However, the culture\u2010based ISO 11731:2017, which is based on counts of colony\u2010forming units per ml (CFU\u00b7ml\n                    <jats:sup>\u20101<\/jats:sup>\n                    ) is still the gold standard for quantification of\n                    <jats:italic>Legionella<\/jats:italic>\n                    species (spp.). As a powerful alternative, we propose real\u2010time monitoring of the growth of\n                    <jats:italic>L<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>pneumophila<\/jats:italic>\n                    using an isothermal microcalorimeter (IMC). Our results demonstrate that, depending on the initial concentration of\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>pneumophila<\/jats:italic>\n                    , detection times of 24\u201348\u00a0h can be reliably achieved. IMC may, therefore, be used as an early warning system for\n                    <jats:italic>L.\u00a0pneumophila<\/jats:italic>\n                    contamination. By replacing only visual detection of growth by a thermal sensor, but otherwise maintaining the standardized protocol of the ISO 11731:2017, the new procedure could easily be incorporated into existing standards. The exact determination of the beginning of metabolic heat is often very difficult because at the beginning of the calorimetric signal the thermal stabilization and the metabolic heat development overlap. Here, we propose a new data evaluation based on the first derivation of the heat flow signal. The improved evaluation method can further reduce detection times and significantly increase the reliability of the IMC approach.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13563","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T03:09:42Z","timestamp":1585105782000},"page":"1262-1272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rapid culture\u2010based detection of\n                    <i>Legionella pneumophila<\/i>\n                    using isothermal microcalorimetry with an improved evaluation method"],"prefix":"10.1111","volume":"13","author":[{"given":"Christian","family":"Fricke","sequence":"first","affiliation":[{"name":"Department of Environmental Microbiology Helmholtz\u2010Centre for Environmental Research \u2013 UFZ Leipzig 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