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Understanding the toxicity mechanisms may allow to lower concentration of metals in the metal\u2010based antimicrobial treatments of crops, and reduce metal content in soil and groundwater. Here, we investigate the interplay between metal efflux systems and the superoxide dismutase (SOD) in the purple bacterium\n                    <jats:italic>Rubrivivax gelatinosus<\/jats:italic>\n                    and other bacteria through analysis of the impact of metal accumulation. Exposure of the Cd\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010efflux mutant \u0394\n                    <jats:italic>cadA<\/jats:italic>\n                    to Cd\n                    <jats:sup>2+<\/jats:sup>\n                    caused an increase in the amount and activity of the cytosolic Fe\u2010Sod SodB, thereby suggesting a role of SodB in the protection against Cd\n                    <jats:sup>2+<\/jats:sup>\n                    . In support of this conclusion, inactivation of\n                    <jats:italic>sodB<\/jats:italic>\n                    gene in the \u0394\n                    <jats:italic>cadA<\/jats:italic>\n                    cells alleviated detoxification of superoxide and enhanced Cd\n                    <jats:sup>2+<\/jats:sup>\n                    toxicity. Similar findings were described in the Cu\n                    <jats:sup>+<\/jats:sup>\n                    \u2010efflux mutant with Cu\n                    <jats:sup>+<\/jats:sup>\n                    . Induction of the Mn\u2010Sod or Fe\u2010Sod in response to metals in other bacteria, including\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas putida<\/jats:italic>\n                    ,\n                    <jats:italic>Vibrio cholera<\/jats:italic>\n                    and\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    , was also shown. Both excess Cd\n                    <jats:sup>2+<\/jats:sup>\n                    or Cu\n                    <jats:sup>+<\/jats:sup>\n                    and superoxide can damage [4Fe\u20104S] clusters. The additive effect of metal and superoxide on the [4Fe\u20104S] could therefore explain the hypersensitive phenotype in mutants lacking SOD and the efflux ATPase. These findings underscore that ROS defence system becomes decisive for bacterial survival under metal excess.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13589","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T06:41:23Z","timestamp":1592548883000},"page":"1515-1529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Additive effects of metal excess and superoxide, a highly toxic mixture in bacteria"],"prefix":"10.1111","volume":"13","author":[{"given":"Anne Soisig","family":"Steunou","sequence":"first","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC) CEA CNRS Universit\u00e9 Paris\u2010Saclay 91198 Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Babot","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC) CEA CNRS 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