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In this study, the\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    bacteriophage D2 (vB_AbaP_D2) was isolated, characterized and sequenced. The endolysin of bacteriophage D2, namely Abtn\u20104, contains an amphipathic helix and was found to have activity against multidrug\u2010resistant Gram\u2010negative strains. By more than 3 log units,\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    were killed by Abtn\u20104 (5\u00a0\u00b5M) in 2\u00a0h. In absence of outer membrane permeabilizers, Abtn\u20104 exhibited broad antimicrobial activity against several Gram\u2010positive and Gram\u2010negative bacteria, such as\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumonia<\/jats:italic>\n                    ,\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    and\n                    <jats:italic>Salmonella<\/jats:italic>\n                    . Furthermore, Abtn\u20104 had the ability to reduce biofilm formation. Interestingly, Abtn\u20104 showed antimicrobial activity against phage\u2010resistant bacterial mutants. Based on these results, endolysin Abtn\u20104 may be a promising candidate therapeutic agent for multidrug\u2010resistant bacterial infections.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13594","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T04:15:19Z","timestamp":1591762519000},"page":"403-418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["The endolysin of the\n                    <i>Acinetobacter baumannii<\/i>\n                    phage vB_AbaP_D2 shows broad antibacterial activity"],"prefix":"10.1111","volume":"14","author":[{"given":"Yuyu","family":"Yuan","sequence":"first","affiliation":[{"name":"School of Bioengineering Dalian University of Technology Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Bioengineering Dalian University of Technology 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