{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:36:40Z","timestamp":1782790600129,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T00:00:00Z","timestamp":1590883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R01HL089315\u201011"],"award-info":[{"award-number":["5R01HL089315\u201011"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["17POST33410497"],"award-info":[{"award-number":["17POST33410497"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["18POST33990223"],"award-info":[{"award-number":["18POST33990223"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006521","name":"School of Medicine, Stanford University","doi-asserted-by":"publisher","award":["Medical Scholars Fellowship Program"],"award-info":[{"award-number":["Medical Scholars Fellowship Program"]}],"id":[{"id":"10.13039\/100006521","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The cyanobacterium\n                    <jats:italic>Synechococcus elongatus<\/jats:italic>\n                    (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar rats intravenously received SE (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a012), sterile saline (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a012) or\n                    <jats:italic>E. coli<\/jats:italic>\n                    lipopolysaccharide (LPS,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a04), and a subset (8 SE, 8 saline) received a repeat injection 4\u00a0weeks later. At baseline, 4\u00a0h, 24\u00a0h, 48\u00a0h, 8\u00a0days and 4\u00a0weeks after injection, clinical assessments, blood cultures, blood counts, lymphocyte phenotypes, liver function tests, proinflammatory cytokines and immunoglobulins were assessed. Across all metrics, SE rats responded comparably to saline controls, displaying no clinically significant immune response. As expected, LPS rats exhibited severe immunological responses. Systemic SE administration does not induce sepsis or toxicity in rats, thereby supporting the safety of cyanobacteria\u2013mammalian symbiotic therapeutics using this organism.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13596","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:25Z","timestamp":1590969625000},"page":"1780-1792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Safety of photosynthetic\n                    <i>Synechococcus elongatus<\/i>\n                    for\n                    <i>in vivo<\/i>\n                    cyanobacteria\u2013mammalian symbiotic therapeutics"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-2760","authenticated-orcid":false,"given":"Kiah M.","family":"Williams","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjay","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Paulsen","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshara D.","family":"Thakore","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Rieck","sequence":"additional","affiliation":[{"name":"Beckman Center for Molecular and Genetic Medicine Stanford University Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley J.","family":"Lucian","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick","family":"Grady","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille E.","family":"Hironaka","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athena J.","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin M.","family":"Farry","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Sook","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Jaatinen","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anahita","family":"Eskandari","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyndsay M.","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"},{"name":"Department of Bioengineering Stanford University Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda N.","family":"Steele","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"},{"name":"Department of Bioengineering Stanford University Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey E.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. Joseph","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Stanford University 300 Pasteur Drive, Falk Cardiovascular Research Building Stanford CA 94305 USA"},{"name":"Department of Bioengineering Stanford University Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,31]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Species specific information: Rat","author":"Ades E.","year":"2019"},{"key":"e_1_2_8_3_1","volume-title":"Molecular Biology of the Cell. Chapter 24: The Adaptive Immune System","author":"Alberts B.","year":"2002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070030101"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0130295"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1586"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142520"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(99)00046-1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-019-2652-7"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstl.1785.0023"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.10.014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.02.016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1603078"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2461-3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-017-0337-7"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.727"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13074217"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.11.049"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.05.012"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/0028825X.2018.1551805"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13118"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000491439"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(02)00180-8"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199803263381304"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.10031014"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.02113.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00211-16"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2016.02.022"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401182"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v6.i9.632"},{"key":"e_1_2_8_31_1","first-page":"3","volume-title":"Recognition and Alleviation of Distress in Laboratory Animals. Recognition and Assessment of Stress and Distress","author":"National Research Council (US) Committee on Recognition and Alleviation of Distress in Laboratory Animals","year":"2008"},{"key":"e_1_2_8_32_1","volume-title":"The Pasteur Culture Collection of Cyanobacteria (PCC)","author":"Institut Pasteur","year":"2019"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1519220112"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1243-4"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00441199"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3A0916-388RR"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00135-16"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-069X-5-6"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101796"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13596","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.13596","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T17:33:17Z","timestamp":1762277597000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.13596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,31]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1111\/1751-7915.13596"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.13596","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,31]]},"assertion":[{"value":"2019-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}