{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:40:24Z","timestamp":1790422824120,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007136","name":"Secretar\u00eda de Estado de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RTC\u20102016\u20104892\u20101"],"award-info":[{"award-number":["RTC\u20102016\u20104892\u20101"]}],"id":[{"id":"10.13039\/501100007136","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The continued development of culturing technologies for the discovery of new molecules from marine microbes is of paramount importance for drug discovery. Coupled with this, the use of the high\u2010throughput approach shows promise for increasing the number of Gram\u2010negative and non\u2010filamentous bacteria cultures that can be surveyed, since they show a lower potential of bioactivity. In this work, we propose a new strategy of high\u2010throughput cultivation of bacteria inspired by a dilution\u2010to\u2010extinction (DTE) methodology for the isolation of, and screening for, new cytotoxic compound producing marine bacteria. A marine sponge tissue was directly used as inoculum and the results were compared with the data obtained through the direct plating isolation method. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC\u2010PCR) genomic fingerprinting indicated the isolation of four bioactive strains, three of them producers of a pederin\u2010like compound, and the fourth one able to synthesize a different compound, still unidentified, rendered by the DTE approach, in comparison with one bioactive strain identified through the plating method. Analyses based on the 16\n                    <jats:italic>S<\/jats:italic>\n                    rRNA gene data showed the existence of two different species belonging to the genus\n                    <jats:italic>Labrenzia<\/jats:italic>\n                    . The efficiency and diversity ratio in the number of isolates and compounds are discussed. In view of the results, the proposed DTE approach proved to be efficient for the isolation of new cytotoxic compounds of marine origin and pave the way for future potential applications.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13679","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T11:38:19Z","timestamp":1603453099000},"page":"241-250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Detection of a pederin\u2010like compound using a dilution\u2010to\u2010extinction\u2010based platform for the isolation of marine bacteria in drug discovery strategies"],"prefix":"10.1111","volume":"14","author":[{"given":"Xulio","family":"Ben\u00edtez","sequence":"first","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4614-3889","authenticated-orcid":false,"given":"Elena G.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paz","family":"Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8161-8299","authenticated-orcid":false,"given":"Fernando","family":"de la Calle","sequence":"additional","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Cuevas","sequence":"additional","affiliation":[{"name":"Research and Development Area PharmaMar S.A. Avda. De los Reyes, 1 P.I. La Mina\u2010Norte Madrid Colmenar Viejo 28770 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00777"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2009.00682.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-5-21"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2019.100622"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NP00092A"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001902"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.8.3878-3885.2002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo2020303"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/284325"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2011.00314.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701693104"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14876"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2010.00188.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12399"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro819"},{"key":"e_1_2_7_17_1","first-page":"1","article-title":"Exploring the cultivable Ectocarpus microbiome","volume":"8","author":"Klein Jan H.","year":"2017","journal-title":"Front Microbiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1365-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.2.795-799.1998"},{"key":"e_1_2_7_20_1","unstructured":"Mohamed R.(2013)Dilution\u2010to\u2010extinction culturing of SAR11 members and other marine bacteria from the Red Sea. Thesis.Saudi Arabia:King Abdullah University of Science and Technology."},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01754-09"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40199-019-00273-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-30194-0_7"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090816-093449"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/B703499G"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.2004.00368.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00917"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2016.05.002"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/md16070244"},{"key":"e_1_2_7_30_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.7b00408"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.59.7.2150-2160.1993"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01203-10"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.39.100185.001541"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00345-12"},{"key":"e_1_2_7_36_1","unstructured":"Swofford D.(2003)PAUP*. 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