{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:55:22Z","timestamp":1778925322569,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0901900"],"award-info":[{"award-number":["2018YFA0901900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771378 and 31800029"],"award-info":[{"award-number":["31771378 and 31800029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Salinomycin, an FDA\u2010approved polyketide drug, was recently identified as a promising anti\u2010tumour and anti\u2010viral lead compound. It is produced by\n                    <jats:italic>Streptomyces albus<\/jats:italic>\n                    , and the biosynthetic gene cluster (\n                    <jats:italic>sal<\/jats:italic>\n                    ) spans over 100\u2009kb. The genetic manipulation of large polyketide gene clusters is challenging, and approaches delivering reliable efficiency and accuracy are desired. Herein, a delicate strategy to enhance salinomycin production was devised and evaluated. We reconstructed a minimized\n                    <jats:italic>sal<\/jats:italic>\n                    gene cluster (mini\u2010cluster) on pSET152 including key genes responsible for tailoring modification, antibiotic resistance, positive regulation and precursor supply. These genes were overexpressed under the control of constitutive promoter P\n                    <jats:italic>\n                      <jats:sub>kasO*<\/jats:sub>\n                    <\/jats:italic>\n                    or P\n                    <jats:italic>\n                      <jats:sub>neo<\/jats:sub>\n                    <\/jats:italic>\n                    . The\n                    <jats:italic>pks<\/jats:italic>\n                    operon was not included in the mini\u2010cluster, but it was upregulated by SalJ activation. After the plasmid pSET152::mini\u2010cluster was introduced into the wild\u2010type strain and a chassis host strain obtained by ribosome engineering, salinomycin production was increased to 2.3\u2010fold and 5.1\u2010fold compared with that of the wild\u2010type strain respectively. Intriguingly, mini\u2010cluster introduction resulted in much higher production than overexpression of the whole\n                    <jats:italic>sal<\/jats:italic>\n                    gene cluster. The findings demonstrated that reconstitution of\n                    <jats:italic>sal<\/jats:italic>\n                    mini\u2010cluster combined with ribosome engineering is an efficient novel approach and may be extended to other large polyketide biosynthesis.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13686","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T11:48:43Z","timestamp":1606996123000},"page":"2356-2368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Reconstitution of a mini\u2010gene cluster combined with ribosome engineering led to effective enhancement of salinomycin production in\n                    <i>Streptomyces albus<\/i>"],"prefix":"10.1111","volume":"14","author":[{"given":"Dong","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxi","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8842-9092","authenticated-orcid":false,"given":"Huarong","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources Institute of Microbiology 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