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However, its titre in the wild\u2010type\n                    <jats:italic>S. pogona<\/jats:italic>\n                    strain is too low to meet the industrial production requirements. Deletion of non\u2010target natural product biosynthetic gene clusters resident in the genome of\n                    <jats:italic>S. pogona<\/jats:italic>\n                    could reduce the consumption of synthetic precursors, thereby promoting the biosynthesis of butenyl\u2010spinosyn. However, it has always been a challenge for scientists to genetically engineer\n                    <jats:italic>S. pogona<\/jats:italic>\n                    . In this study, the Latour gene knockout system (linear DNA fragment recombineering system) was established in\n                    <jats:italic>S. pogona<\/jats:italic>\n                    . Using the Latour system, a hybrid NRPS\u2010T1PKS cluster (\u02dc20\u2009kb) which was responsible for phthoxazolin biosynthesis was efficiently deleted in\n                    <jats:italic>S. pogona<\/jats:italic>\n                    . The resultant mutant\n                    <jats:italic>S. pogona<\/jats:italic>\n                    \u2010\u0394\n                    <jats:italic>ura4\u2010<\/jats:italic>\n                    \u0394c14 exhibited an extended logarithmic phase, increased biomass and a lower glucose consumption rate. Importantly, the production of butenyl\u2010spinosyn in\n                    <jats:italic>S. pogona<\/jats:italic>\n                    \u2010\u0394\n                    <jats:italic>ura4\u2010<\/jats:italic>\n                    \u0394c14 was increased by 4.72\u2010fold compared with that in the wild\u2010type strain. qRT\u2010PCR analysis revealed that phthoxazolin biosynthetic gene cluster deletion could promote the expression of the butenyl\u2010spinosyn biosynthetic gene cluster. Furthermore, a TetR family transcriptional regulatory gene that could regulate the butenyl\u2010spinosyn biosynthesis has been identified from the phthoxazolin biosynthetic gene cluster. Because dozens of natural product biosynthetic gene clusters exist in the genome of\n                    <jats:italic>S. pogona<\/jats:italic>\n                    , the strategy reported here will be used to further promote the production of butenyl\u2010spinosyn by deleting other secondary metabolite synthetic gene clusters.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13694","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T09:23:47Z","timestamp":1604136227000},"page":"2369-2384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Deletion of a hybrid NRPS\u2010T1PKS biosynthetic gene cluster via Latour gene knockout system in\n                    <i>Saccharopolyspora pogona<\/i>\n                    and its effect on butenyl\u2010spinosyn biosynthesis and growth development"],"prefix":"10.1111","volume":"14","author":[{"given":"Jie","family":"Rang","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory for Microbial Molecular Biology State Key Laboratory of Development Biology of Freshwater Fish College of Life Science Hunan Normal University Changsha 410081 China"},{"name":"Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (MOE of China) National &amp; Local Joint Engineering Laboratory for New Petro\u2010chemical Materials and Fine Utilization of Resources College of Chemistry and Chemical Engineering Hunan Normal University Changsha 410081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunlong","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Microbial Molecular Biology State Key Laboratory of Development Biology of Freshwater Fish College of Life Science Hunan Normal University Changsha 410081 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