{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:08:23Z","timestamp":1786752503412,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T00:00:00Z","timestamp":1604707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772105\uff0c"],"award-info":[{"award-number":["31772105\uff0c"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31600118"],"award-info":[{"award-number":["31600118"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    As a pathogenic fungus,\n                    <jats:italic>Aspergillus flavus<\/jats:italic>\n                    can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen\u2010activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In this study, the roles of membrane mucin Msb2 were explored in\n                    <jats:italic>A. flavus<\/jats:italic>\n                    by the application of gene disruption. The deletion of\n                    <jats:italic>msb2<\/jats:italic>\n                    gene (\u0394\n                    <jats:italic>msb2<\/jats:italic>\n                    ) caused defects in vegetative growth, sporulation and sclerotia formation when compared to WT and complement strain (\u0394\n                    <jats:italic>\n                      msb2\n                      <jats:sup>C<\/jats:sup>\n                    <\/jats:italic>\n                    ) in\n                    <jats:italic>A. flavus<\/jats:italic>\n                    . Using thin\u2010layer chromatography (TLC) and high\u2010performance liquid chromatography (HPLC) analysis, it was found that deletion of\n                    <jats:italic>msb2<\/jats:italic>\n                    down\u2010regulated aflatoxin B\n                    <jats:sub>1<\/jats:sub>\n                    (AFB\n                    <jats:sub>1<\/jats:sub>\n                    ) synthesis and decreased the infection capacity of\n                    <jats:italic>A. flavus<\/jats:italic>\n                    . Consistently, Msb2 responds to cell wall stress and osmotic stress by positively regulating the phosphorylation of MAP kinase. Notably, \u0394\n                    <jats:italic>msb2<\/jats:italic>\n                    mutant exhibited cell wall defect, and it was more sensitive to inhibitor caspofungin when compared to WT and \u0394\n                    <jats:italic>\n                      msb2\n                      <jats:sup>C<\/jats:sup>\n                    <\/jats:italic>\n                    . Taking together, these results revealed that Msb2 plays key roles in morphological development process, stresses adaptation, secondary metabolism and pathogenicity in fungus\n                    <jats:italic>A. flavus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13701","type":"journal-article","created":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T10:13:52Z","timestamp":1604744032000},"page":"628-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus\n                    <i>Aspergillus flavus<\/i>"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0629-8947","authenticated-orcid":false,"given":"Ling","family":"Qin","sequence":"first","affiliation":[{"name":"Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province Key Laboratory of 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