{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:04:42Z","timestamp":1762272282245,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:00:00Z","timestamp":1607126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    is the most common sexually transmitted pathogen globally, causing serious health problems and representing a burden on public health. A new variant of\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    (nvCT) that carries mutations (C1514T, C1515T and G1523A) in the 23S rRNA gene has eluded detection in Aptima Combo 2 assays. This has led to false negatives in diagnostics tests and poses a challenge for\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    diagnostics on a global level. In this study, we developed a simple and cost\u2010effective assay to identify\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    , with a potential application to screen for nvCT. We developed a screening assay based on high\u2010resolution melting (HRM), targeting the 23S rRNA gene and cryptic plasmid. To evaluate the performance of the assay, 404 archived\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    DNA specimens and 570 extracted clinical specimens were analysed. Our HRM assay not only identified\n                    <jats:italic>C. trachomatis<\/jats:italic>\n                    in clinical specimens, but also correctly differentiated nvCT carrying C1514T, C1515T and G1523A mutations from the wild\u2010type. We observed no cross\u2010reactions with other clinically related agents, and the limit of detection was 11.26 (95% CI; 7.61\u201331.82) copies per reaction. Implementation of this screening assay could reduce detection times and costs for C. trachomatis diagnoses, and facilitate increased research on the presence and monitoring of nvCT.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13724","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T08:22:33Z","timestamp":1607156553000},"page":"668-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A molecular screening assay to identify\n                    <i>Chlamydia trachomati<\/i>\n                    s and distinguish new variants of\n                    <i>C. trachomatis<\/i>\n                    from wild\u2010type"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0391-1742","authenticated-orcid":false,"given":"Leshan","family":"Xiu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"},{"name":"Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamei","family":"Li","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"},{"name":"Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"},{"name":"Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yizhun","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Chronic Disease Control Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaling","family":"Zeng","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Chronic Disease Control Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Chronic Disease Control Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7959-5286","authenticated-orcid":false,"given":"Junping","family":"Peng","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"},{"name":"Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.44.1900219"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01083-17"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/epirev\/mxz007"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06194.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01969-19"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7070187"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13043"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1409.080153"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7080227"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.028076-0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.42.1900592"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2007.026435"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1084-8592(97)80024-0"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00035-15"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms4030025"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01446-09"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1586\/erm.09.84"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.22.1900298"},{"key":"e_1_2_10_20_1","first-page":"E061109.2","article-title":"A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests","volume":"11","author":"Ripa T.","year":"2006","journal-title":"Euro Surveill"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.38.1900557"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1071\/SH18205"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01709-12"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178653"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32834204d5"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.24.1900354"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.26.1900370"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05148-7"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.2807\/esm.12.04.00699-en"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.036830-0"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S219580"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz395"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13724","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.13724","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.13724","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:50:58Z","timestamp":1762271458000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.13724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,5]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/1751-7915.13724"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.13724","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"type":"print","value":"1751-7915"},{"type":"electronic","value":"1751-7915"}],"subject":[],"published":{"date-parts":[[2020,12,5]]},"assertion":[{"value":"2020-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}