{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:48:50Z","timestamp":1792108130934,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K19875"],"award-info":[{"award-number":["18K19875"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Since methylotrophic yeasts such as\n                    <jats:italic>Ogataea methanolica<\/jats:italic>\n                    can use methanol as a sole carbon feedstock, they could be applied to produce valuable products from methanol, a next\u2010generation energy source synthesized from natural gases, using genetic engineering tools. In this study, metabolite profiling of\n                    <jats:italic>O<\/jats:italic>\n                    .\n                    <jats:italic>methanolica<\/jats:italic>\n                    was conducted under glucose (Glc) and low and high methanol (L\u2010 and H\u2010MeOH) conditions to show the adaptation mechanism to a H\u2010MeOH environment. The yeast strain responded not only to the presence of methanol but also to its concentration based on the growth condition. Under H\u2010MeOH conditions,\n                    <jats:italic>O. methanolica<\/jats:italic>\n                    downregulated the methanol utilization, glycolytic pathway and alcohol oxidase (AOD) isozymes and dihydroxyacetone synthase (DAS) expression compared with L\u2010MeOH\u2010grown cells. However, levels of energy carriers, such as ATP, were maintained to support cell survival. In H\u2010MeOH\u2010grown cells, reactive oxygen species (ROS) levels were significantly elevated. Along with increasing ROS levels, ROS scavenging system expression was significantly increased in H\u2010MeOH\u2010grown cells. Thus, we concluded that formaldehyde and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , which are products of methanol oxidation by AOD isozymes in the peroxisome, are overproduced in H\u2010MeOH\u2010grown cells, and excessive ROS derived from these cells is generated in the cytosol, resulting in upregulation of the antioxidant system and downregulation of the methanol\u2010utilizing pathway to suppress overproduction of toxic intermediates.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13811","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T12:13:43Z","timestamp":1620044023000},"page":"1512-1524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Metabolic regulation adapting to high methanol environment in the methylotrophic yeast\n                    <i>Ogataea methanolica<\/i>"],"prefix":"10.1111","volume":"14","author":[{"given":"Hao\u2010Liang","family":"Cai","sequence":"first","affiliation":[{"name":"The United Graduate School of Agricultural Science Gifu University 1\u20101 Yanagido Gifu 501\u20101193 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryohei","family":"Doi","sequence":"additional","affiliation":[{"name":"The Graduate School of Natural Sciences and Technologies 1\u20101 Yanagido Gifu 501\u20101193 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Shimada","sequence":"additional","affiliation":[{"name":"The United Graduate School of Agricultural Science Gifu University 1\u20101 Yanagido Gifu 501\u20101193 Japan"},{"name":"The Graduate School of Natural Sciences and Technologies 1\u20101 Yanagido Gifu 501\u20101193 Japan"},{"name":"The Faculty of Applied Biological Sciences 1\u20101 Yanagido Gifu 501\u20101193 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