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Apart from the lung, SARS\u2010CoV\u20102 was detected in multiple organs in severe cases. Less is known on organ tropism in patients developing mild or no symptoms, and some of such patients might be missed in symptom\u2010indicated swab testing. Here, we tested and validated several approaches and selected the most reliable RT\u2010PCR protocol for the detection of SARS\u2010CoV\u20102 RNA in patients\u2019 routine diagnostic formalin\u2010fixed and paraffin\u2010embedded (FFPE) specimens available in pathology, to assess (i) organ tropism in samples from COVID\u201019\u2010positive patients, (ii) unrecognized cases in selected tissues from negative or not\u2010tested patients during a pandemic peak, and (iii) retrospectively, pre\u2010pandemic lung samples. We identified SARS\u2010CoV\u20102 RNA in seven samples from confirmed COVID\u201019 patients, in two gastric biopsies, one small bowel and one colon resection, one lung biopsy, one pleural resection and one pleural effusion specimen, while all other specimens were negative. In the pandemic peak cohort, we identified one previously unrecognized COVID\u201019 case in tonsillectomy samples. All pre\u2010pandemic lung samples were negative. In conclusion, SARS\u2010CoV\u20102 RNA detection in FFPE pathology specimens can potentially improve surveillance of COVID\u201019, allow retrospective studies, and advance our understanding of SARS\u2010CoV\u20102 organ tropism and effects.<\/jats:p>","DOI":"10.1111\/1751-7915.13828","type":"journal-article","created":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T12:24:05Z","timestamp":1621167845000},"page":"1627-1641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["SARS\u2010CoV\u20102 RNA screening in routine pathology specimens"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5260-8494","authenticated-orcid":false,"given":"Saskia","family":"von Stillfried","sequence":"first","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen 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M.","family":"Buhl","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen Germany"},{"name":"Electron Microscopy Facility RWTH Aachen University Hospital Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Maurer","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadina","family":"Ortiz\u2010Br\u00fcchle","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5883-3580","authenticated-orcid":false,"given":"Peter","family":"Celec","sequence":"additional","affiliation":[{"name":"Institute of Virology Biomedical Research Center Slovak Academy of Sciences Bratislava Slovakia"},{"name":"Institute of Pathophysiology Faculty of Medicine Comenius University Bratislava Slovakia"},{"name":"Department of Molecular Biology Faculty of Natural Sciences Comenius University Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1566-7129","authenticated-orcid":false,"given":"Barbara M.","family":"Klinkhammer","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4798-2908","authenticated-orcid":false,"given":"Dickson W.L.","family":"Wong","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Cacchi","sequence":"additional","affiliation":[{"name":"Institute of Pathology RWTH Aachen University Hospital Aachen 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